Anomalous properties of water in macromolecular gels
| Autor: | Ehud Ben-Hur, Michel Paardekooper, John Van Steveninck, Tom M.A.R. Dubbelman |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Properties of water
Elution Size-exclusion chromatography Temperature Thiourea Biophysics Analytical chemistry Galactose Water Biochemistry Polyethylene Glycols Molecular Weight Solutions chemistry.chemical_compound Adsorption chemistry Sephadex Chemical physics Chromatography Gel Molecule Molecular Biology Vicinal Macromolecule |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1115:96-100 |
| ISSN: | 0304-4165 |
| Popis: | Low molecular weight solutes often exhibit elution characteristics on gel filtration columns which deviate from ideal behaviour. In many previous studies this anomalous behaviour was attributed to the existence of extremely narrow pores in the gel, inaccessible even to very small solute molecules, to explain Kd values lower than unity. Kd values of small solutes higher than unity were usually ascribed to adsorption of the solute to the gel matrix. In the present paper several observations are presented that contradict these suggestions. Experimental evidence indicates that with small solute molecules Kd values differing from unity can be fully explained by the anomalous properties of vicinal water layers at the gel matrix-water interface. |
| Databáze: | OpenAIRE |
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