The synthesis and fate of glycodelin in human ovary during folliculogenesis
| Autor: | Jym Tse, Markku Seppälä, Wsb Yeung, R Koistinen, Philip C.N. Chiu, Hannu Koistinen, Y.Q. Yao, Kai-Fai Lee |
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| Rok vydání: | 2002 |
| Předmět: |
endocrine system
Embryology medicine.medical_specialty Ovary Pregnancy Proteins Biology Binding Competitive Iodine Radioisotopes Andrology Follicle Ovarian Follicle Internal medicine Genetics medicine Humans RNA Messenger Ovarian follicle Molecular Biology Glycoproteins Granulosa Cells Glycodelin urogenital system Obstetrics and Gynecology Cell Biology Follicular fluid Cumulus oophorus medicine.anatomical_structure Endocrinology Reproductive Medicine Theca Theca Cells Female Folliculogenesis Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 8:142-148 |
| ISSN: | 1460-2407 |
| Popis: | The ontogeny of glycodelin in human ovarian follicles during folliculogenesis was studied. Glycodelin immunoreactivity began to be detected in the granulosa cells and thecal cells of late secondary follicles. Immunoreactivity was also found in both the luteinized granulosa cells and cumulus cells obtained from women undergoing the assisted reproduction treatment. However, only the luteinized granulosa cells, and not the cumulus cells, expressed glycodelin mRNA. Results also showed that the cumulus cells took up radiolabelled glycodelin and partially deglycosylated some of it. Glycodelin (and a partially deglycolsylated form of glycoldelin) appeared to complex with two cytoplasmic or membrane components of the cumulus cells. The data also demonstrated that ZIF-1, a glycoprotein isolated from human follicular fluid, was immunologically similar to glycodelin. In conclusion, we suggest that glycodelin is synthesized in the granulosa cells of ovarian follicles at late secondary follicle stage. It then may be released into the follicular fluid from where it is taken up and partially modified by the cumulus cells. |
| Databáze: | OpenAIRE |
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