A Critical Evaluation of In Vitro Hesperidin 2S Bioavailability in a Model Combining Luminal (Microbial) Digestion and Caco-2 Cell Absorption in Comparison to a Randomized Controlled Human Trial
| Autor: | John Van Camp, Judit Pitart, Stefan Voorspoels, Griet Jacobs, Guy Smagghe, Evelien Van Rymenant, Charlotte Grootaert, Sam Possemiers, Bouke Salden, Bart Noten |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Colon Surface Properties Flavonoid Models Biological Antioxidants 03 medical and health sciences Hesperidin chemistry.chemical_compound Glucuronides Double-Blind Method In vivo medicine Humans Food science chemistry.chemical_classification Flavonoids 030109 nutrition & dietetics Plant Extracts Hydrolysis Hesperetin food and beverages Small intestine Bioavailability Gastrointestinal Microbiome Kinetics 030104 developmental biology medicine.anatomical_structure chemistry Intestinal Absorption Caco-2 Polyphenol Cinnamates Fruit Dietary Supplements Digestion Female Caco-2 Cells Food Science Biotechnology Citrus sinensis |
| Zdroj: | Molecular nutritionfood research. 62(8) |
| ISSN: | 1613-4133 |
| Popis: | Scope: Bioavailability strongly determines polyphenol bioactivity, and is strongly influenced by food matrix, enzymatic and microbial degradation, and gastrointestinal absorption. To avoid human trials for pre-screening of polyphenol bioavailability, studies have focused on in vitro model development. Nevertheless, their predictive value for bioavailability can be questioned. Method and results: We used the orange flavonoid hesperidin 2S to validate a model combining digestion in the simulator of the human intestinal microbial ecosystem (SHIME) and Caco-2 cell transport, with a human intervention study. In vitro, hesperidin was resistant to degradation in the stomach and small intestine, but was rapidly deconjugated on reaching the proximal colon. Extensive and colon-region-specific degradation to smaller phenolics was observed. Hydrocaffeic and dihydroisoferulic acid accumulated in proximal, and hydroferulic acid in distal colon. Caco-2 transport was the highest for dihydroisoferulic acid. In humans, plasma and urine hesperetin-glucuronide levels increased significantly, whereas the impact on small phenolics was limited. Conclusions: In the combined in vitro model, smaller phenolics strongly accumulated, whereas in humans, hesperetin conjugates were the main bioavailable compounds. Future in vitro model development should focus on simulating faster polyphenol absorption and elimination of smaller phenolics to improve their predictive value of in vivo polyphenol bioavailability. |
| Databáze: | OpenAIRE |
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