Characterization of Inwardly Rectifying K+ Conductance across the Basolateral Membrane of Rat Tracheal Epithelia
| Autor: | Young-Jin Son, Tae Ho Hwang, Sang-Hoon Shin, Jin-Han Yoon, Jin-Sup Jung, Sung-Han Lee, Hye-Jeong Lee, Il Yun, Jin-Sook Jeong, Hee-Sook Lee |
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| Rok vydání: | 2001 |
| Předmět: |
Cell Membrane Permeability
Potassium Channels Blotting Western Molecular Sequence Data Respiratory System Biophysics Respiratory Mucosa Biochemistry Membrane Potentials Cell polarity Potassium Channel Blockers Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Potassium Channels Inwardly Rectifying Molecular Biology Cells Cultured Ion channel Epithelial polarity Chromatography Chemistry Myocardium Cell Membrane Electric Conductivity Kir2.1 Cell Polarity Conductance Cell Biology Apical membrane Hyperpolarization (biology) Immunohistochemistry Potassium channel Rats Trachea Nasal Mucosa Potassium Calcium Ion Channel Gating |
| Zdroj: | Biochemical and Biophysical Research Communications. 288:914-920 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.5831 |
| Popis: | The rat primary cultured-airway monolayer has been an excellent model for deciphering the ion channel after nystatin permeabilization of its basolateral or apical membrane. Inwardly rectifying K(+) currents were characterized across the basolateral membrane in symmetrical HCO(-)(3)-free high K(+) Ringer's solution (125 mM) in this study. The potency of K(+) channel inhibitors against K(+) conductance was Ba(2+) (IC(50) = 5 microM) > Cs(+) (IC(50) = 2 mM) >> glybenclamide (IC(50) > 5 mM) >> TEA (IC(50) >> 100 mM). The application of basolateral Cs(+) changed K(+) conductance into an oscillating current, and its frequency (holding voltage = -100 mV) increased with increase in concentration of basolateral Cs(+) (0.05-5 mM) and in degree of hyperpolarization. Addition of basolateral Cs(+) blocked inward current strongly at -100 mV and hardly at all at -60 mV, giving a sharp curvature to the I-V relation of the IRK current. RT-PCR, Western blotting, and immunohistochemical analyses showed that Kir2.1 might be present in basolateral membrane of tracheal epithelia and plasma membrane of pulmonary alveolar cells. |
| Databáze: | OpenAIRE |
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