In Vitro and in Vivo Evaluation of Lactoferrin-Conjugated Liposomes as a Novel Carrier to Improve the Brain Delivery
| Autor: | Su-Tang Lo, Jem-Mau Lo, Te-Wei Lee, Feng-Yun J. Huang, Wan-Jou Chen, Wan-Yu Lee |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Biodistribution
Pharmacology Blood–brain barrier Article Catalysis lcsh:Chemistry Inorganic Chemistry In vivo medicine Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy 99mTc Liposome lactoferrin PEGylated liposome blood-brain barrier business.industry Organic Chemistry General Medicine In vitro Computer Science Applications medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 Nanoparticles for drug delivery to the brain PEGylation business Drug carrier |
| Zdroj: | International Journal of Molecular Sciences; Volume 14; Issue 2; Pages: 2862-2874 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 14, Iss 2, Pp 2862-2874 (2013) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms14022862 |
| Popis: | In this study, lactoferrin-conjugated PEGylated liposomes (PL), a potential drug carrier for brain delivery, was loaded with radioisotope complex, 99mTc labeled N,N-bis(2-mercaptoethyl)-N',N'-diethylethylenediamine (99mTc-BMEDA) for in vitro and in vivo evaluations. The hydrophilicity of liposomes was enhanced by PEGylation which was not an ideal brain delivery system for crossing the blood brain barrier (BBB). With the modification of a brain-targeting ligand, lactoferrin (Lf), the PEGylated liposome (PL) might become a potential brain delivery vehicle. In order to test the hypothesis in vitro and in vivo, 99mTc-BMEDA was loaded into the liposomes as a reporter with or without Lf-conjugation. The mouse brain endothelia cell line, bEnd.3 cells, was cultured to investigate the potential uptake of liposomes in vitro. The in vivo uptake by the mouse brain of the liposomes was detected by tissue biodistribution study. The results indicated that Lf-conjugated PEGylated liposome showed more than three times better uptake efficiency in vitro and two-fold higher of brain uptake in vivo than PEGlyated liposome. With the success of loading the potential Single Photon Emission Tomography (SPECT) imaging probe, 99mTc-BMEDA, Lf-PL might serve as a promising brain delivery system for loading diagnostics or therapeutics of various brain disorders. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|