Can Human Recombinant Epidermal Growth Factor Improve Ischemia and Induce Healing of Anastomosis in an Experimental Study in a Rabbit Model?
| Autor: | Mustafa Özer, Rahman Şenocak, Orhan Kozak, Metin Uyanık, Armagan Gunal, Zafer Kilbas, Şahin Kaymak |
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| Rok vydání: | 2016 |
| Předmět: |
Male
EGF Family of Proteins endocrine system medicine.medical_specialty Recombinant Epidermal Growth Factor Angiogenesis Ischemia Anastomotic Leak Anastomosis Random Allocation 03 medical and health sciences 0302 clinical medicine Ileum Anastomotic leaks Intestinal walls medicine Animals Humans Intestinal Mucosa Wound Healing business.industry Anastomosis Surgical medicine.disease Recombinant Proteins Surgery Hydroxyproline 030220 oncology & carcinogenesis Rabbit model 030211 gastroenterology & hepatology Collagen Rabbits business Wound healing Injections Intraperitoneal |
| Zdroj: | Journal of Investigative Surgery. 30:101-109 |
| ISSN: | 1521-0553 0894-1939 |
| Popis: | Anastomotic leaks following intestinal operations may cause devastating effects on patients. Ischemia may also occur at the intestinal walls in the presence of strangulations. In this study, we examined the effects of human recombinant (Hr)-epidermal growth factor (EGF) given at a single intramural dose into the intestinal walls and daily intraperitoneal cavity on ischemia and the healing process of anastomosis.Sixteen male New Zeland white rabbits were randomly divided into four groups (n = 4 in each group). In Group 1, two different segments of ileum were identified and, then, transected and the free ends were sutured each other. In the other groups, ischemia was induced by ligating the mesenteric vascular arcade. After the ischemic induction, Group 2 received intramural injections of %0.9 saline, Group 3 received intramural injections of a single dose of EGF, and Group 4 received intramural and intraperitoneal injections of EGF. Bursting pressures and tissue hydroxyproline levels were analyzed. Necrosis, fibroblastic activity, collagen deposition and neovascularization were also studied.The mean levels of bursting pressures in Group 4 (148.6 ± 25.3 mmHg) were higher than Group 2 (70 ± 21.5 mmHg) (p = 0.001). The mean level of bursting pressures was not statistically significant between Group 1 (170.1 ± 35 mmHg) and Group 4 (p = 0.073). Hydroxyproline levels in Group 2 were lower than Groups 3 and 4. There was a statistically significant difference in the mucosal ischemia, mucosal healing and degree of adhesion, but not in the mural anastomotic healing among the groups.Intramural injection with daily intraperitoneal administration of low-dose EGF enhances the bursting pressure and collagen accumulation in ischemic anastomosis, improving many histological variables associated with ischemic intestinal anastomosis. |
| Databáze: | OpenAIRE |
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