Dye-Mediated Photo-Oxidation Biomaterial Fixation: Analysis of Bioinductivity and Mechanical Properties of Bovine Pericardium for Use in Cardiac Surgery

Autor: Kristina Jeon, Ali Fatehi Hassanabad, Guoqi Teng, Vishnu Vasanthan, Sean Kang, Karl T. Wagner, Paul W.M. Fedak, Simranjit S. Pattar
Jazyk: angličtina
Rok vydání: 2021
Předmět:
medicine.medical_specialty
Bovine pericardium
Biocompatibility
Photochemistry
QH301-705.5
Biocompatible Materials
Article
Catalysis
vasculogenesis
Inorganic Chemistry
Extracellular matrix
Paracrine signalling
Vasculogenesis
medicine
Animals
Humans
Cardiac Surgical Procedures
Biology (General)
Physical and Theoretical Chemistry
Coloring Agents
QD1-999
Molecular Biology
Spectroscopy
Fixation (histology)
Bioprosthesis
Chemistry
Organic Chemistry
biomaterial
Biomaterial
cardiac fibroblast
General Medicine
Fibroblasts
Biomechanical Phenomena
Extracellular Matrix
Computer Science Applications
Cardiac surgery
tissue fixation
Cross-Linking Reagents
myocardial infarction
cardiovascular system
Cattle
cardiac reconstruction
Pericardium
post-infarct cardiac repair
cardiac surgery
Biomedical engineering
dye-mediated photo-oxidation
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 19
International Journal of Molecular Sciences, Vol 22, Iss 10768, p 10768 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms221910768
Popis: Extracellular matrix bioscaffolds can influence the cardiac microenvironment and modulate endogenous cellular mechanisms. These materials can optimize cardiac surgery for repair and reconstruction. We investigated the biocompatibility and bioinductivity of bovine pericardium fixed via dye-mediated photo-oxidation on human cardiac fibroblast activity. We compared a dye-mediated photo-oxidation fixed bioscaffold to glutaraldehyde-fixed and non-fixed bioscaffolds reported in contemporary literature in cardiac surgery. Human cardiac fibroblasts from consenting patients were seeded on to bioscaffold materials to assess the biocompatibility and bioinductivity. Human cardiac fibroblast gene expression, secretome, morphology and viability were studied. Dye-mediated photo-oxidation fixed acellular bovine pericardium preserves human cardiac fibroblast phenotype and viability
and potentiates a pro-vasculogenic paracrine response. Material tensile properties were compared with biomechanical testing. Dye-mediated photo-oxidation fixed acellular bovine pericardium had higher compliance compared to glutaraldehyde-fixed bioscaffold in response to tensile force. The biocompatibility, bioinductivity, and biomechanical properties of dye-mediated photo-oxidation fixed bovine pericardium demonstrate its feasibility as a bioscaffold for use in cardiac surgery. As a fixed yet bioinductive solution, this bioscaffold demonstrates enhanced compliance and retains bioinductive properties that may leverage endogenous reparative pathways. Dye-mediated photo-oxidation fixed bioscaffold warrants further investigation as a viable tool for cardiac repair and reconstruction.
Databáze: OpenAIRE
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