Time course of the tilorone-induced lysosomal accumulation of sulphated glycosaminoglycans in cultured fibroblasts
| Autor: | Manfred W. Ziegenhagen, Jens Fischer, Renate Lüllmann-Rauch, Freia Bispinck |
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| Rok vydání: | 1998 |
| Předmět: |
Time Factors
Dermatan Sulfate Tilorone Biology Toxicology Pathology and Forensic Medicine Glycosaminoglycan Pathogenesis Cornea Adjuvants Immunologic In vivo medicine Lysosomal storage disease Animals Fibroblast Cells Cultured Chondroitin Sulfates Cell Biology General Medicine Fibroblasts medicine.disease In vitro medicine.anatomical_structure Biochemistry Cell culture Cattle Heparitin Sulfate Lysosomes medicine.drug |
| Zdroj: | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 50(4-6) |
| ISSN: | 0940-2993 |
| Popis: | Summary Dicationic amphiphilic drugs such as the immunomodulator tilorone {2,7-bis-[2-(diethylamino)ethoxy]fluoren-9-one} are accumulated in lysosomes and disturb the degradation of sulphated glycosaminoglycans (GAGs) thus leading to generalized lysosomal GAG storage (mainly dermatan sulphate) in vivo and in cultured cells. In the present study, the time course of the tilorone-induced GAG storage was determined in cultured bovine corneal fibroblasts by a radio-chemical approach and by morphological examination. In contrast to the rapid lysosomal accumulation of the drug as reported previously, it took approximately 42 h to reach 50% of the GAG storage obtained after 96 h. This is thought to be due to the fact that the temporal development of storage of undigested GAGs depends on the natural delivery of GAGs towards the lysosomal apparatus. |
| Databáze: | OpenAIRE |
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