Inhibition of intercellular communication: An in vitro assay for detecting potential tumour promoters

Autor:	J. Fentem, Michael Balls, A. Cooper
Rok vydání:	1994
Předmět:	Tumour promoters General Medicine Biology Toxicology Cell junction In vitro chemistry.chemical_compound medicine.anatomical_structure Investigation methods Biochemistry chemistry In vivo Immunology medicine Phorbol esters Keratinocyte Intracellular
Zdroj:	Toxicology in Vitro. 8:535-537
ISSN:	0887-2333
DOI:	10.1016/0887-2333(94)90009-4
Popis:	The effects of non-cytotoxic concentrations of 13 chemicals on gap junctional intercellular communication (GJIC) in human keratinocytes and WB 344 cells were determined using a scrape-labelling method. The chemicals were selected according to their reported tumour-promoting effects in either the skin or liver, or their known skin irritancy potential. The in vitro assay was able to discriminate between genotoxic and non-genotoxic chemicals and, with keratinocyte cultures, ranked correctly a series of phorbol esters with regard to their reported tumour-promoting potencies in vivo. The data obtained support the suggestion that measurement of the inhibition of GJIC in vitro may provide a means to distinguish substances that have different tumour-promoting potencies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7932faa4234ce5da5907427ff2eb4d1 https://doi.org/10.1016/0887-2333(94)90009-4 Zobrazit plný text záznamu