A detailed linkage map of lettuce based on SSAP, AFLP and NBS markers
Autor: | Anker P. Sørensen, Wendy van’t Westende, Hans C. M. Den Nijs, Clemens C. M. van de Wiel, C. Gerard van der Linden, Naeem H. Syed, Rudie Antonise, Danny A. P. Hooftman, Andrew J. Flavell |
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Přispěvatelé: | Experimental Plant Systematics (IBED, FNWI) |
Rok vydání: | 2005 |
Předmět: |
Genetic Markers
disease resistance molecular markers DNA Plant Genetic Linkage s-sap Lactuca Locus (genetics) Retrotransposon Genome diversity analysis Chromosomes Plant Gene flow Genetic linkage PRI Biodiversiteit en Veredeling assisted selection Genetics Crosses Genetic transpositional activity biology Terminal Repeat Sequences Lactuca serriola food and beverages barley Chromosome Mapping General Medicine genetic diversity Lettuce biology.organism_classification Random Amplified Polymorphic DNA Technique PRI Biodiversity and Breeding Long Interspersed Nucleotide Elements ty1-copia group retrotransposons Amplified fragment length polymorphism polymorphisms Agronomy and Crop Science Genome Plant Biotechnology Microsatellite Repeats |
Zdroj: | Theoretical and Applied Genetics, 112(3), 517-527. Springer Verlag Theoretical and Applied Genetics, 112(3), 517-527 Theoretical and Applied Genetics 112 (2006) 3 |
ISSN: | 0040-5752 |
Popis: | Molecular markers based upon a novel lettuce LTR retrotransposon and the nucleotide binding site-leucine-rich repeat (NBS-LRR) family of disease resistance-associated genes have been combined with AFLP markers to generate a 458 locus genetic linkage map for lettuce. A total of 187 retrotransposon-specific SSAP markers, 29 NBS-LRR markers and 242 AFLP markers were mapped in an F2 population, derived from an interspecific cross between a Lactuca sativa cultivar commonly used in Europe and a wild Lactuca serriola isolate from Northern Europe. The cross has been designed to aid efforts to assess gene flow from cultivated lettuce into the wild in the perspective of genetic modification biosafety. The markers were mapped in nine major and one minor linkage groups spanning 1,266.1 cM, with an average distance of 2.8 cM between adjacent mapped markers. The markers are well distributed throughout the lettuce genome, with limited clustering of different marker types. Seventy-seven of the AFLP markers have been mapped previously and cross-comparison shows that the map from this study corresponds well with the previous linkage map. |
Databáze: | OpenAIRE |
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