Cellular Effects of In Vitro -Digested Aluminum Nanomaterials on Human Intestinal Cells
| Autor: | Dajana Lichtenstein, Holger Sieg, Peter Laux, Albert Braeuning, Claudia Kästner, Harald Jungnickel, Linda Böhmert, Andreas F. Thünemann, Valérie Fessard, Alfonso Lampen, Jutta Tentschert, Andreas Luch, Benjamin-Christoph Krause |
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| Přispěvatelé: | Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), ANR-13-IS10-0005,SolNanoTOX,Détermination de facteurs de toxicité au niveau intestinal et hépatique de deux nanoparticules de taille similaire utilisées en alimentation et en emballage : Recherches in vitro et in vivo sur l'absorption et les mécanismes impliqués.(2013) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell
human intesinal cell cellule intestinale humaine 010501 environmental sciences 01 natural sciences Artificial digestion Transcriptome nanomatériau 03 medical and health sciences test in-vitro medicine toxicité General Materials Science Viability assay sécurité des aliments Inductively coupled plasma mass spectrometry 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Chemistry aluminium Human gastrointestinal tract public health toxicity toxicologie In vitro in-vitro test food safety medicine.anatomical_structure Caco-2 santé publique [SDV.TOX]Life Sciences [q-bio]/Toxicology Biophysics tract intestinal nanomaterial intestinal tract nanotoxicology nanotoxicologie toxicology |
| Zdroj: | ACS Applied Nano Materials ACS Applied Nano Materials, American Chemical Society, 2020, 3 (3), pp.2246-2256. ⟨10.1021/acsanm.9b02354⟩ |
| ISSN: | 2574-0970 |
| Popis: | International audience; Aluminum (Al) can be taken up from food, packaging, or the environment and thus reaches the human gastrointestinal tract. Its toxic potential after oral uptake is still discussed. The fate of different solid and ionic Al species during the passage through the digestive tract is the focus of this research, as well as the cellular effects caused by these different Al species. The present study combines the physicochemical processing of three recently studied Al species (metallic Al0, mineral Al2O3, and soluble AlCl3) in artificial digestion fluids with in vitro cell systems for the human intestinal barrier. Inductively coupled plasma mass spectrometry (ICP-MS) and small-angle X-ray scattering (SAXS) methods were used to characterize the Al species in the artificial digestion fluids and in cell culture medium for proliferating and differentiated intestinal Caco-2 cells. Cytotoxicity testing and cellular impedance measurements were applied to address the effects of digested Al species on cell viability and cell proliferation. Microarray-based transcriptome analyses and quantitative real-time PCR were conducted to obtain a deeper insight into cellular mechanisms of action and generated indications for cellular oxidative stress and an influence on xenobiotic metabolism, connected with alterations in associated signaling pathways. These cellular responses, which were predominantly caused by formerly ionic Al species and only at very high concentrations, were not impacted by artificial digestion. A two-directional conversion of Al between ionic species and solid particles occurred throughout all segments of the gastrointestinal tract, as evidenced by the presence of nanoscaled particles. Nevertheless, this presence did not increase the toxicity of the respective Al species. |
| Databáze: | OpenAIRE |
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