Pharmacological evaluation for anticancer and immune activities of a novel polysaccharide isolated from Boletus speciosus Frost
| Autor: | Ting Xu, Wanru Hou, Yichun Zeng, Hongqing Zhu, Xiang Ding, Fang Wang, J.C. Zhong, Bo Song, Yiling Hou, Jian Li, Ting Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Male Cancer Research Lipopolysaccharide Nitric Oxide Synthase Type II Antineoplastic Agents Capsules Crystallography X-Ray Nitric Oxide Biochemistry Nitric oxide Mice chemistry.chemical_compound Immune system Polysaccharides Cell Line Tumor Neoplasms Genetics Animals Humans RNA Messenger Cell Shape Molecular Biology Cell Proliferation Immunity Cellular Mice Inbred BALB C biology Gene Expression Profiling Immunity Molecular biology In vitro Gene Expression Regulation Neoplastic Nitric oxide synthase Oncology chemistry Cell culture Apoptosis Macrophages Peritoneal biology.protein Cytokines Molecular Medicine Growth inhibition Agaricales |
| Zdroj: | Molecular Medicine Reports. 9:1337-1344 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2014.1976 |
| Popis: | The fungal polysaccharides have been revealed to exhibit a variety of biological activities, including antitumor, immune-stimulation and antioxidation activities. In the present study, the immune and anticancer activities of a novel polysaccharide, BSF-A, isolated from Boletus speciosus Frost was investigated. The inhibitory rate of S180 tumors in mice treated with 40 mg/kg BSF-A reached 62.449%, which was the highest rate from the three doses administered; this may be comparable to mannatide. The antitumor activity of BSF-A is commonly considered to be a consequence of the stimulation of the cell-mediated immune response, as it may significantly promote the macrophage cells in the dose range of 100-400 µg/ml in vitro. The levels of the cytokines, IL-6, IL-1β and TNF-α, and nitric oxide, induced by BSF-A treatment at varying concentrations in the macrophage cells were similar to the levels in the cells treated with lipopolysaccharide. There was weak expression of the TNF-α, IL-6, IL-1β and inducible nitric oxide synthase mRNA in the untreated macrophages, but this increased significantly in a dose-dependent manner in the BSF-A-treated cells. BSF-A also had a time- and dose-dependent effect on the growth inhibition of the Hep-2 cells, with the concentration of 400 µg/ml having the highest inhibitory rate. A quantitative PCR array analysis of the gene expression profiles indicated that BSF-A had anticancer activities that affected cell apoptosis in the Hep-2 cells. The results obtained in the present study indicated that the purified polysaccharide of Boletus speciosus Frost is a potential source of natural anticancer substances. |
| Databáze: | OpenAIRE |
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