Docosahexaenoic and eicosapentaenoic fatty acids differentially regulate glucose and fatty acid metabolism in L6 rat skeletal muscle cells
| Autor: | Rolando B. Ceddia, Glen Katsnelson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Docosahexaenoic Acids Physiology Glucose uptake Anti-Inflammatory Agents Palmitates 030209 endocrinology & metabolism AMP-Activated Protein Kinases Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Phosphorylation Glycogen synthase Muscle Skeletal chemistry.chemical_classification Muscle Cells biology Fatty acid metabolism Carnitine O-Palmitoyltransferase Chemistry food and beverages Skeletal muscle Lipid metabolism Cell Biology Lipid Metabolism Eicosapentaenoic acid Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha 3. Good health Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Glucose Eicosapentaenoic Acid Docosahexaenoic acid biology.protein lipids (amino acids peptides and proteins) Polyunsaturated fatty acid |
| Zdroj: | American journal of physiology. Cell physiology. 319(6) |
| ISSN: | 1522-1563 |
| Popis: | The objective of this study was to investigate whether the n-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) can directly regulate glucose and fat metabolism in skeletal muscle besides exerting anti-inflammatory effects. To accomplish this, L6 skeletal muscle cells were treated with 50 µM of either DHA or EPA for 1, 3, and 5 days. Here, we report that basal and insulin-stimulated rates of glucose uptake, glycogen synthesis, protein kinase B (AKT), and glycogen synthase kinase 3 (GSK3) phosphorylation were not affected by DHA or EPA. However, glucose and palmitate oxidation were consistently elevated by DHA treatment, whereas EPA only increased this variable transiently. Similarly, only DHA caused significant and sustained increases in AMP-activated protein kinase (AMPK) phosphorylation and protein levels of carnitine-palmitoyl transferase-1b (CPT1b) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) in skeletal muscle cells. DHA also caused a larger anti-inflammatory effect than EPA in these cells. In conclusion, besides exerting anti-inflammatory effects, DHA and EPA directly regulated glucose and fat metabolism in skeletal muscle cells, although DHA was more effective in doing so than EPA. Thus, by directly enhancing glucose and fat oxidation, DHA may increase glucose disposal and reduce intramyocellular lipid accumulation. |
| Databáze: | OpenAIRE |
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