Cromolyn ameliorates acute and chronic injury in a rat lung transplant model
| Autor: | Zoie E. Holzknecht, Matthew G. Hartwig, Jui-Chih Chang, Jason H. Leung, Soman N. Abraham, Shu S. Lin, R. Duane Davis, Tao Tang, William Parker |
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| Rok vydání: | 2013 |
| Předmět: |
Pulmonary and Respiratory Medicine
Graft Rejection Male medicine.medical_specialty Pathology medicine.medical_treatment Acute Lung Injury obliterative bronchiolitis Gastroenterology Rats Inbred WKY Article Postoperative Complications Internal medicine Cromolyn Sodium Medicine Animals Anti-Asthmatic Agents Mast Cells Saline Bronchiolitis Obliterans Lung gastric fluid Medicine(all) Transplantation aspiration Gastric fluid business.industry Lung Injury Chronic injury medicine.disease Mast cell Allografts Rats Disease Models Animal medicine.anatomical_structure Bronchiolitis Cytokines Surgery Chemokines pulmonary allograft Cardiology and Cardiovascular Medicine business Allotransplantation Lung Transplantation |
| Zdroj: | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 33(7) |
| ISSN: | 1557-3117 |
| Popis: | BackgroundMast cells have been associated with obliterative bronchiolitis (OB) in human pulmonary allografts, although their role in the development of OB remains unknown.MethodsIn this study, we evaluated the role of mast cells in pulmonary allograft rejection using an orthotopic rat pulmonary allograft model that utilizes chronic aspiration of gastric fluid to reliably obtain OB. Pulmonary allograft recipients (n = 35) received chronic aspiration of gastric fluid with (n = 10) and without (n = 16) treatment with a mast cell membrane stabilizer, cromolyn sodium, or chronic aspiration with normal saline (n = 9) as a control.ResultsThe acute graft injury associated with long ischemic time in the model (6 hours total ischemic time; typical acute graft injury rate ~30%) was apparently blocked by cromolyn, because peri-operative mortality associated with the acute graft injury was not observed in any of the animals receiving cromolyn (p = 0.045). Further, the rats receiving cromolyn developed significantly fewer OB lesions than those treated with gastric fluid alone (p < 0.001), with a mean reduction of 46% of the airways affected.ConclusionsThese findings provide impetus for further studies aimed at elucidating the effects of cromolyn and the role of mast cells in pulmonary allotransplantation. |
| Databáze: | OpenAIRE |
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