Activation of guinea pig alveolar macrophages by endothelin-1
| Autor: | V. Millul, G. Bereziat, O. Gillardeaux, B. Dugas, J. Masliah, Elisabeth Boichot, Vincent Lagente, Jean Michel Mencia-Huerta, Pierre Braquet |
|---|---|
| Přispěvatelé: | Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), UPRES-A CNRS 7079, Centre National de la Recherche Scientifique ( CNRS ), CHU, Université Pierre et Marie Curie - Paris 6 ( UPMC ) |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Metabolite Guinea Pigs Enzyme-Linked Immunosorbent Assay In Vitro Techniques 030226 pharmacology & pharmacy Reuptake Guinea pig 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine [ CHIM.ORGA ] Chemical Sciences/Organic chemistry Internal medicine Macrophages Alveolar medicine Animals Incubation Pharmacology Arachidonic Acid [CHIM.ORGA]Chemical Sciences/Organic chemistry Endothelins Macrophage Activation Endothelin 1 N-Formylmethionine Leucyl-Phenylalanine Thromboxane B2 Endocrinology chemistry Biochemistry Luminescent Measurements Luminol Arachidonic acid Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cardiovascular Pharmacology Journal of Cardiovascular Pharmacology, 1991, 17 Suppl 7, pp.S233-5. ⟨10.1097/00005344-199100177-00067⟩ Journal of Cardiovascular Pharmacology, Lippincott, Williams & Wilkins, 1991, 17 Suppl 7, pp.S233-5 |
| ISSN: | 0160-2446 |
| DOI: | 10.1097/00005344-199100177-00067⟩ |
| Popis: | International audience; Endothelin-1 (ET-1) induced a concentration- and time-dependent increase in arachidonic acid release from guinea pig alveolar macrophages, a maximum being reached at 1 nM ET-1. Regarding the time-course study, maximal release of arachidonic acid was observed after 30 s of incubation with ET-1 and then followed by a marked decrease, suggesting a reuptake of free arachidonic acid. ET-1 (0.1-10 nM) induced a concentration- and time-dependent release of TXB2, the stable metabolite of TXA2, evaluated by an ELISA technique. Maximum release was also obtained with 1 nM ET-1 but only after a 1-min incubation period. By contrast, ET-1 (1 pM-1 microM) did not stimulate the release of superoxide anion at any time point of the kinetic study. These results suggests that ET-1 may activate guinea pig alveolar macrophages, probably through a mechanism involving the arachidonic acid pathway. |
| Databáze: | OpenAIRE |
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