Development of a model to demonstrate photosensitizer-mediated viral inactivation in blood
| Autor: | Tufaro F, Bartel Dl, Levy Jg, Neyndorff Hc |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Neutral red
Radiation-Sensitizing Agents Lysis Erythrocytes Time Factors Light viruses Immunology Hemolysis Virus Vesicular stomatitis Indiana virus chemistry.chemical_compound Immunology and Allergy Humans Photosensitizer Hematoporphyrin Derivative Incubation Whole blood Red Cell biology Hematology biology.organism_classification Virology Molecular biology Hematoporphyrins Blood chemistry Photochemotherapy Vesicular stomatitis virus Virus Activation |
| Zdroj: | Transfusion. 30(6) |
| ISSN: | 0041-1132 |
| Popis: | A model has been developed to demonstrate the use of photodynamic treatment (PDT) to eradicate viral contaminants from donated blood and blood products. Whole blood, spiked with vesicular stomatitis virus (VSV), was treated with the photosensitizer benzoporphyrin derivative-monoacid ring A (BPD-MA). After light activation of BPD-MA, a neutral red dye uptake assay was carried out to determine virus inactivation. Various drug incubation times and light intensities were tested as well as red cell lysis and distribution of VSV in blood. At BPD-MA concentrations between 2 and 4 micrograms per mL in whole blood, up to 10(7) VSV were inactivated. Several photosensitizers were also tested with this model to determine their relative efficacy in viral inactivation. |
| Databáze: | OpenAIRE |
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