An integrated process for the production of 1,3-propanediol, lactate and 3-hydroxypropionic acid by an engineered Lactobacillus reuteri
| Autor: | Pandiaraj Suppuram, Ramalingam Subramanian, Gopi Gopal Ramakrishnan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Glycerol Limosilactobacillus reuteri 010501 environmental sciences 3-Hydroxypropionic acid 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Industrial Microbiology Aldehyde Reductase 010608 biotechnology Production (economics) Food science 1 3-Propanediol Lactic Acid Transgenes Molecular Biology Biotransformation 0105 earth and related environmental sciences biology Escherichia coli Proteins Organic Chemistry Food grade General Medicine biology.organism_classification Lactobacillus reuteri Glucose chemistry Metabolic Engineering Batch Cell Culture Techniques Propylene Glycols Scientific method Yield (chemistry) Fermentation Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 83(4) |
| ISSN: | 1347-6947 |
| Popis: | The process economy of food grade 1,3-propanediol (1,3-PD) production by GRAS organisms like Lactobacillus reuteri (L. reuteri), is negatively impacted by the low yield and use of expensive feedstocks. In order to improve the process economy, we have developed a multiproduct process involving the production of three commercially important chemicals, namely, 1,3-PD, lactate and 3-Hydroxypropionic acid (3-HP), by engineered L. reuteri. The maximum 1,3-PD and lactate titer of 41 g/L and 31 g/L, with a volumetric productivity of 1.69 g/L/h and 0.67 g/L/h were achieved, respectively. The maximum 3-HP titer of 5.2 g/L with a volumetric productivity of 1.3 g/L/h, was obtained by biotransformation using cells recovered from the repeated fed-batch process. The volumetric productivity of 1,3-PD obtained in this study is the highest ever reported for this organism. Further cost reduction can be achieved by using waste feedstocks like milk whey, biomass hydrolysate, and crude glycerol. |
| Databáze: | OpenAIRE |
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