Induction of IFN-gamma in macrophages by lipopolysaccharide
| Autor: | Marion J. Fultz, Sheila A. Barber, Stefanie N. Vogel, Carl W. Dieffenbach |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide medicine.medical_treatment Immunology Population Molecular Sequence Data Bone Marrow Cells Biology Polymerase Chain Reaction Natural killer cell chemistry.chemical_compound Interferon-gamma Mice Immune system medicine Escherichia coli Immunology and Allergy Macrophage Animals RNA Messenger education Cells Cultured education.field_of_study Mice Inbred BALB C Mice Inbred C3H Base Sequence Macrophages General Medicine Molecular biology Mice Inbred C57BL Blotting Southern medicine.anatomical_structure Cytokine chemistry Microscopy Fluorescence Cell culture Polyinosinic:polycytidylic acid Macrophages Peritoneal Female |
| Zdroj: | International immunology. 5(11) |
| ISSN: | 0953-8178 |
| Popis: | In this paper we report that macrophages can be stimulated to express detectable levels of IFN-gamma-specific mRNA. Macrophages from lipopolysaccharide (LPS)-responsive, C3H/OuJ mice are induced by LPS to increase steady-state levels of IFN-gamma-specific mRNA, while those from LPS-hyporesponsive C3H/HeJ mice are not. This interstrain variation is apparently the result of LPS-specific signal differences since macrophages derived from both Lpsn and Lpsd mouse strains are able to produce comparable levels of IFN-gamma-specific mRNA following stimulation with polyinosinic-polycytidylic acid. The identity of the cell type responsible for this IFN-gamma message appears to be the macrophage as IFN-gamma-specific mRNA was also detectable following T and natural killer cell depletion, in the LPS-stimulated RAW 264.7 cell line, and in a homogeneous population of mature macrophages propagated in vitro by stimulation of bone marrow progenitors with recombinant colony stimulating factor-1. Immunofluorescent staining of fixed and permeabilized LPS-stimulated macrophages confirmed the presence of immunoreactive IFN-gamma protein. The possible significance of IFN-gamma production by macrophages is discussed in the context of normal macrophage differentiation as well as the inflammatory immune response. |
| Databáze: | OpenAIRE |
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