Theileria-infected cell line from an African buffalo (Syncerus caffer)
| Autor: | Abdalla A. Latif, Fred T. Potgieter, Antoinette I. Josemans, Ronel Pienaar, Natasha E. Rambritch, Otto Koekemoer, Erich Zweygarth, John Fraser Putterill |
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| Rok vydání: | 2009 |
| Předmět: |
Buffaloes
DNA Ribosomal Peripheral blood mononuclear cell 18S ribosomal RNA law.invention law Theileria parasitic diseases RNA Ribosomal 18S Animals Cluster Analysis Gene Cells Cultured Phylogeny Polymerase chain reaction Genetics Differential centrifugation General Veterinary biology Genes rRNA Sequence Analysis DNA General Medicine DNA Protozoan Ribosomal RNA biology.organism_classification Molecular biology Infectious Diseases Cell culture Insect Science Leukocytes Mononuclear Cattle Parasitology RNA Protozoan |
| Zdroj: | Parasitology Research. 105:579-581 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-009-1467-0 |
| Popis: | Mononuclear cells were isolated from the peripheral blood of a buffalo infected with a Theileria sp. using density gradient centrifugation, and the cells were put into culture flasks covered by a monolayer of bovine endothelial cells. Twenty days after culture initiation, cells containing macroschizonts were detected in Giemsa-stained smears. The first subculture was carried out on day 45 of culture propagation. Subsequently, infected cells were subcultured twice a week, and each time 1 to 2 x 10(6) per milliliter cells were harvested. DNA was extracted from culture material and a partial polymerase chain reaction amplification of the 18S ribosomal RNA (rRNA) gene was carried out using Theileria genus-specific primers. Sequence data and phylogenetic analysis using the 18S rRNA gene indicated a close relationship to Theileria sp. buffalo, previously described in literature. Here, the first successful attempt to establish a macroschizont-infected lymphoblastoid cell line of Theileria sp. (buffalo) from an African buffalo is described. |
| Databáze: | OpenAIRE |
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