Effects of time-of-day on the concentration of defined excitatory and inhibitory amino acids in the cerebrospinal fluid of rats: a microdialysis study
| Autor: | Liliana Carmona-Aparicio, Noemí Cárdenas-Rodríguez, Rosalinda Guevara-Guzmán, Francisco Estrada-Rojo, Virginia Arriaga-Ávila, Adan Perez-Arredondo, Elvia Coballase-Urrutia, Luz Navarro |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Alanine chemistry.chemical_classification medicine.medical_specialty Microdialysis Chemistry Organic Chemistry Clinical Biochemistry Glutamate receptor Biochemistry Circadian Rhythm Rats Amino acid Glutamine Glutamatergic Endocrinology Internal medicine Glycine medicine Excitatory postsynaptic potential Animals Amino Acids Rats Wistar |
| Zdroj: | Amino Acids. 53:1597-1607 |
| ISSN: | 1438-2199 0939-4451 |
| Popis: | Amino acid neurotransmitters are responsible for many physiological and pathological processes, and their cerebral concentrations respond to external influences such as the light-dark cycle and to the synthesis, release, and recapture rhythms and form part of the biochemical relationships derived from excitatory-inhibitory (E/I), glutamine-glutamate sum (GLX), glutamatergic processing (glutamine-glutamate ratio) and excitotoxic indexes. The changes in these variables during a 24-h period (1 day) are important because they allow organisms to adapt to external stimuli and form part of physiological processes. Under pathological conditions, the damage produced by acute events may depend on diurnal variations. Therefore, it is important to analyze the extracellular levels of amino acids as well as the above-mentioned indexes over a 24-h period. We focused on determining the cerebrospinal fluid levels of different amino acid neurotransmitters, and the E/I, GLX, glutamatergic processing and excitotoxic indexes, determined by microdialysis over a 24-h cycle. Our results showed significant changes during the 24-h light/dark cycle. Specifically, we found increments in the levels of glutamate (325%), GABA (550%), glutamine (300%), glycine (194%), alanine (304%) and the GLX index (263%) throughout the day, and the maximum levels of glutamate, glutamine, glycine, and alanine were obtained during the last period of the light period. In conclusion, the concentration of some amino acid neurotransmitters and the GLX index show variations depending on the light-dark cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink