Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota
| Autor: | Panagiotis Georgiadis, Athina Boulaka, Georgia Saxami, Georgios Koutrotsios, Adamantini Kyriacou, Paraschos Christodoulou, Georgios I. Zervakis, Maria I. Zervou, Vasiliki Pletsa, Marigoula Vlassopoulou, Georgios Bekiaris, Evdokia K. Mitsou |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
in vitro fermentation
Magnetic Resonance Spectroscopy beta-Glucans medicine.medical_treatment Pharmaceutical Science medicine.disease_cause Analytical Chemistry genoprotection Feces 0302 clinical medicine Drug Discovery Food science chemistry.chemical_classification 0303 health sciences biology Chemistry food and beverages Basidiomycota Chemistry (miscellaneous) 030220 oncology & carcinogenesis Metabolome Molecular Medicine β-glucans psychological phenomena and processes animal structures edible mushrooms Polysaccharide Protective Agents Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry medicine Humans Metabolomics Pleurotus eryngii Physical and Theoretical Chemistry 030304 developmental biology Glucan Biological Products NMR-based metabolomics Prebiotic fungi Organic Chemistry Fungi biology.organism_classification nervous system Fermentation Pleurotus ostreatus Caco-2 Cells Agaricales faecal microbiota Genotoxicity |
| Zdroj: | Molecules, Vol 25, Iss 3554, p 3554 (2020) Molecules Volume 25 Issue 15 |
| ISSN: | 1420-3049 |
| Popis: | A variety of bioactive compounds, constituents of edible mushrooms, in particular &beta glucans, i.e., a group of &beta d-glucose polysaccharides abundant in the fungal cell walls, have been linked to immunomodulating, anticancer and prebiotic activities. The aim of the study was the investigation of the genoprotective effects of edible mushrooms produced by Pleurotus eryngii, Pleurotus ostreatus and Cyclocybe cylindracea (Basidiomycota). Mushrooms from selected strains of the species mentioned above were fermented in vitro using faecal inocula from healthy volunteers. The cytotoxic and anti-genotoxic properties of the fermentation supernatants (FSs) were investigated in Caco-2 human colon adenocarcinoma cells. The FSs were cytotoxic in a dose-dependent manner. Non-cytotoxic concentrations were used for the genotoxicity studies, which revealed that mushrooms&rsquo FSs have the ability to protect Caco-2 cells against tert-butyl hydroperoxide (t-BOOH), a known genotoxic agent. Their global metabolic profiling was assessed by 1H-NMR spectroscopy. A total of 37 metabolites were identified with the use of two-dimensional (2D) homo- and hetero-nuclear NMR experiments. Multivariate data analysis monitored the metabolic variability of gut microbiota and probed to biomarkers potentially associated with the health-promoting effects of edible mushrooms. |
| Databáze: | OpenAIRE |
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