Dual role of oxidative stress-JNK activation in autophagy and apoptosis induced by nickel oxide nanoparticles in human cancer cells
| Autor: | Rizwan Wahab, Yik-Lam Cho, Weifeng He, Han-Ming Shen, Javed Ahmad, Quaiser Saquib, Yi Ren, Boon-Huat Bay, Hayden Weng Siong Tan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
0301 basic medicine education Apoptosis Oxidative phosphorylation Mitochondrion medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Nickel Physiology (medical) Neoplasms medicine Autophagy Cytotoxic T cell Humans Cytotoxicity health care economics and organizations Chemistry technology industry and agriculture respiratory system Cell biology Oxidative Stress 030104 developmental biology Nanoparticles Reactive Oxygen Species Flux (metabolism) 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Free radical biologymedicine. 153 |
| ISSN: | 1873-4596 |
| Popis: | Nickel oxide nanoparticles (NiO-NPs) are an important group of nanoparticles with increasing applications in many aspects of industry. At present, there is evidence demonstrating the cytotoxic characteristics of NiO-NPs, while the involvement of autophagy in the cytotoxicity of NiO-NPs has not been reported. In this study, we aimed to study the role of autophagy in the cytotoxicity of NiO-NPs and the underlying regulatory mechanisms. First, we provided evidence that NiO-NPs induce autophagy in human cancer cells. Second, we found that the enhanced autophagic flux by NiO-NPs via the generation of intracellular reactive oxygen species (ROS) from mitochondria and the subsequent activation of the JNK pathway. Third, we demonstrated that the activation of JNK is a main force in mediating NiO-NPs-induced apoptosis. Finally, we demonstrated that the autophagic response plays an important protective role against the cytotoxic effect of NiO-NPs. Therefore, this study identifies the dual role of oxidative stress-JNK activation in the biological effects of NiO-NPs via promoting autophagy and mediating apoptosis. Understanding the protective role of autophagy and the underlying mechanism is important for the potential application of NiO-NPs in the biomedical industry. |
| Databáze: | OpenAIRE |
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