Four-Week Repeated Intravenous Dose Toxicity of Self-Assembled-Micelle Inhibitory RNA-Targeting Amphiregulin in Mice
| Autor: | Han Oh Park, Tae-Rim Kim, Sunghwan Kim, Jong-Choon Kim, Jun Hong Park, In-Hyeon Kim, Je-Oh Lim, Hyeon-Young Kim, In-Chul Lee, Sung-Il Yun |
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| Rok vydání: | 2021 |
| Předmět: |
Male
No-observed-adverse-effect level Pharmacology Toxicology Inhibitory postsynaptic potential Amphiregulin Micelle Self assembled 03 medical and health sciences 0302 clinical medicine Animals RNA Small Interfering Micelles 030304 developmental biology Intravenous dose Mice Inbred ICR No-Observed-Adverse-Effect Level 0303 health sciences Chemistry Toxicity Tests Subacute 030220 oncology & carcinogenesis Reagent Injections Intravenous Toxicity Nanoparticles Female |
| Zdroj: | International Journal of Toxicology. 40:453-465 |
| ISSN: | 1092-874X 1091-5818 |
| DOI: | 10.1177/10915818211031241 |
| Popis: | The present study investigated the potential subchronic toxicity of self-assembled-micelle inhibitory RNA-targeting amphiregulin (SAMiRNA-AREG) in mice. The test reagent was administered once-daily by intravenous injection for 4 weeks at 0, 100, 200, or 300 mg/kg/day doses. Additional recovery groups (vehicle control and high dose groups) were observed for a 2-week recovery period. During the test period, mortality, clinical signs, body weight, food consumption, ophthalmology, urinalysis, hematology, serum biochemistry, gross pathology, organ weight, and histopathology were examined. An increase in the percentages of basophil and large unstained cells was observed in the 200 and 300 mg/kg/day groups of both sexes. In addition, the absolute and relative weights of the spleen were higher in males given 300 mg/kg/day relative to the concurrent controls. However, these findings were considered of no toxicological significance because the changes were minimal, were not accompanied by other relevant results (eg, correlating microscopic changes), and were not observed at the end of the 2-week recovery period indicating recovery of the findings. Based on the results, SAMiRNA-AREG did not cause treatment-related adverse effects at dose levels of up to 300 mg/kg/day in mice after 4-week repeated intravenous doses. Under these conditions, the no-observed-adverse-effect level of the SAMiRNA-AREG was ≥300 mg/kg/day in both sexes and no target organs were identified. |
| Databáze: | OpenAIRE |
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