Three-day-old human unfertilized oocytes after in vitro fertilization/intracytoplasmic sperm injection can be activated by calcium ionophore a23187 or strontium chloride and develop to blastocysts
| Autor: | Ying Liu, Guo-qing Ren, Chan-wei Jia, Li Wang, Lan Yu, Wei Li, Xiao-jie Han, Ming-hui Liu, Shu-yu Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Time Factors medicine.medical_treatment Parthenogenesis Strontium chloride Ionophore chemistry.chemical_element Calcium Biology Intracytoplasmic sperm injection Andrology chemistry.chemical_compound medicine Humans Blastocyst Sperm Injections Intracytoplasmic reproductive and urinary physiology Calcimycin Cells Cultured In vitro fertilisation Pronucleus urogenital system Embryo Cell Biology Anatomy Original Articles Calcium Ionophores medicine.anatomical_structure chemistry Strontium embryonic structures Oocytes Female Developmental Biology Biotechnology |
| Zdroj: | Cellular reprogramming. 16(4) |
| ISSN: | 2152-4998 |
| Popis: | Our objective was to observe the effectiveness of the calcium ionophore A23187 or strontium chloride on the activation and subsequent embryonic development of 3-day-old human unfertilized oocytes after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). A total of 279 3-day-old unfertilized oocytes after IVF or ICSI were randomized to be activated by the calcium ionophore A23187 (n=138) or strontium chloride (n=141). The activated oocytes were cultured in vitro for 3-5 days. Activation rate, pronucleus formation, cleavage rate, and developmental potential of parthenotes during culture were evaluated. A total of 170 unfertilized oocytes were activated; 65 developed to cleavage stage, 19 developed to greater than the eight-cell stage, and five blastocysts were obtained. The activation rate of the calcium ionophore A23187 group was higher than that of the strontium chloride group (75.4% and 46.8%, respectively; p |
| Databáze: | OpenAIRE |
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