Endoscopic application of mussel-inspired phenolic chitosan as a hemostatic agent for gastrointestinal bleeding: A preclinical study in a heparinized pig model

Autor: Keumyeon Kim, Haeshin Lee, Gawon Song, Moon Sue Lee, Mi-Young Koh, Abdullah Ozguer Yeniova, In Kyung Yoo, Joo Young Cho
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Physiology
Swine
Catechols
Drug Evaluation
Preclinical
Endoscopic mucosal resection
Gastroenterology
Vascular Medicine
Hemostatics
Endoscopy
Gastrointestinal
Animal Cells
Pig Models
Medicine and Health Sciences
Omeprazole
Ulcers
Hemostatic Agent
Multidisciplinary
Hematology
Animal Models
Experimental Organism Systems
Medicine
Cellular Types
Gastrointestinal Hemorrhage
medicine.drug
Research Article
medicine.medical_specialty
Gastrointestinal bleeding
Science
Immune Cells
Immunology
Antigen-Presenting Cells
Argon plasma coagulation
Surgical and Invasive Medical Procedures
Hemorrhage
Research and Analysis Methods
Signs and Symptoms
Internal medicine
Tissue Repair
medicine
Animals
Hemostasis
Chitosan
business.industry
Heparin
Biology and Life Sciences
Anticoagulants
Endoscopy
Cell Biology
medicine.disease
Bivalvia
Disease Models
Animal
Animal Studies
Rebamipide
Clinical Medicine
business
Physiological Processes
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 5, p e0251145 (2021)
ISSN: 1932-6203
Popis: Marine mussels secrete adhesive proteins to attach to solid surfaces. These proteins contain phenolic and basic amino acids exhibiting wet adhesion properties. This study used a mussel-inspired hemostatic polymer, chitosan-catechol, to treat gastrointestinal bleeding caused by endoscopic mucosal resection in a heparinized porcine model. We aimed to evaluate the hemostatic efficacy and short-term safety of this wet adhesive chitosan-catechol. We used 15 heparinized pigs. Four iatrogenic bleeding ulcers classified as Forrest Ib were created in each pig using an endoscopic mucosal resection method. One ulcer in each pig was untreated as a negative control (no-treatment group). The other three ulcers were treated with gauze (gauze group), argon plasma coagulation (APC group), and chitosan-catechol hemostatic agent (CHI-C group) each. The pigs were sacrificed on Days 1, 5, and 10, and histological examination was performed (n = 5 per day). Rapid hemostasis observed at 2 min after bleeding was 93.3% (14/15) in the CHI-C group, 6.7% (1/15) in the no-treatment group, 13.3% (2/15) in the gauze group, and 86.7% (13/15) in the APC group. No re-bleeding was observed in the CHI-C group during the entire study period. However, a few re-bleeding cases were observed on Day 1 in the no-treatment, gauze, and APC groups and on Day 5 in the gauze and APC groups. On histological analysis, the CHI-C group showed the best tissue healing among the four test groups. Considering the results, chitosan-catechol is an effective hemostatic material with reduced re-bleeding and improved healing.
Databáze: OpenAIRE
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