Cyclic olefin copolymer as an X-ray compatible material for microfluidic devices
| Autor: | Oliva Saldanha, Andrew Wittmeier, Chiara Cassini, Eleonora Perego, Sarah Köster, Manfred Burghammer, Ana Diaz, Manuela Denz, Heidi Spears, Clément Y. J. Hémonnot, Gerrit Brehm |
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| Rok vydání: | 2018 |
| Předmět: |
Fabrication
Materials science Scattering 010401 analytical chemistry Microfluidics Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology General Chemistry Cyclic olefin copolymer 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 0104 chemical sciences Kapton Rendering (computer graphics) Controllability chemistry.chemical_compound chemistry 0210 nano-technology |
| Zdroj: | Lab on a Chip. 18:171-178 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c7lc00824d |
| Popis: | The combination of microfluidics and X-ray methods attracts a lot of attention from researchers as it brings together the high controllability of microfluidic sample environments and the small length scales probed by X-rays. In particular, the fields of biophysics and biology have benefited enormously from such approaches. We introduce a straightforward fabrication method for X-ray compatible microfluidic devices made solely from cyclic olefin copolymers. We benchmark the performance of the devices against other devices including more commonly used Kapton windows and obtain data of equal quality using small angle X-ray scattering. An advantage of the devices presented here is that no gluing between interfaces is necessary, rendering the production very reliable. As a biophysical application, we investigate the early time points of the assembly of vimentin intermediate filament proteins into higher-order structures. This weakly scattering protein system leads to high quality data in the new devices, thus opening up the way for numerous future applications. |
| Databáze: | OpenAIRE |
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