Development of a novel nannochloropsis strain with enhanced violaxanthin yield for large‐scale production
| Autor: | Ae Jin Ryu, Dae-Hyun Cho, Joohyun Yun, Jin-Ho Yun, Sujin Lee, Su-Bin Park, Saehae Choi, Dong Yun Choi, Ji Won Kim, Hee-Sik Kim, Yong Jae Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Mutant lcsh:QR1-502 Bioengineering Gamma‐ray irradiation Xanthophylls 01 natural sciences Applied Microbiology and Biotechnology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Pigment Bioreactor Microalgae Food science Biomass Carotenoid 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Strain (chemistry) Random mutagenesis Research biology.organism_classification chemistry Yield (chemistry) visual_art Mutation visual_art.visual_art_medium Nannochloropsis oceanica Violaxanthin Transcriptome Nannochloropsis Stramenopiles 010606 plant biology & botany Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 20, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1475-2859 |
| Popis: | Background Nannochloropsis is a marine microalga that has been extensively studied. The major carotenoid produced by this group of microalgae is violaxanthin, which exhibits anti-inflammatory, anti-photoaging, and antiproliferative activities. Therefore, it has a wide range of potential applications. However, large-scale production of this pigment has not been much studied, thereby limiting its industrial application. Results To develop a novel strain producing high amount of violaxanthin, various Nannochloropsis species were isolated from seawater samples and their violaxanthin production potential were compared. Of the strains tested, N. oceanica WS-1 exhibited the highest violaxanthin productivity; to further enhance the violaxanthin yield of WS-1, we performed gamma-ray-mediated random mutagenesis followed by colorimetric screening. As a result, Mutant M1 was selected because of its significant higher violaxanthin content and biomass productivity than WS-1 (5.21 ± 0.33 mg g− 1 and 0.2101 g L− 1 d− 1, respectively). Subsequently, we employed a 10 L-scale bioreactor to confirm the large-scale production potential of M1, and the results indicated a 43.54 % increase in violaxanthin production compared with WS-1. In addition, comparative transcriptomic analysis performed under normal light condition identified possible mechanisms associated with remediating photo-inhibitory damage and other key responses in M1, which seemed to at least partially explain enhanced violaxanthin content and delayed growth. Conclusions Nannochloropsis oceanica mutant (M1) with enhanced violaxanthin content was developed and its physiological characteristics were investigated. In addition, enhanced production of violaxanthin was demonstrated in the large-scale cultivation. Key transcriptomic responses that are seemingly associated with different physiological responses of M1 were elucidated under normal light condition, the details of which would guide ongoing efforts to further maximize the industrial potential of violaxanthin producing strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink