Nicotinamide mononucleotide production by fructophilic lactic acid bacteria
| Autor: | Nobuyuki Yoshida, Yuji Tokimoto, Miyako Yoshino, Koji Nishikawa, Hideaki Idogaki, Kana Iijima, Hideo Dohra, Kazane Sugiyama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Niacinamide Science Nicotinamide phosphoribosyltransferase Pyridinium Compounds Fructose Article Applied microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactobacillales Escherichia coli Nicotinamide Phosphoribosyltransferase Nicotinamide Mononucleotide Nicotinamide mononucleotide chemistry.chemical_classification Multidisciplinary biology biology.organism_classification Yeast Lactic acid 030104 developmental biology Enzyme chemistry Biochemistry Nicotinamide riboside Medicine 030217 neurology & neurosurgery Lactic acid fermentation Bacteria Leuconostoc Biotechnology |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Nicotinamide mononucleotide (NMN), an intermediate in nicotinamide adenine dinucleotide biosynthesis, is recently attracting much attention for its pharmacological and anti-aging efficacies. However, current commercial products containing NMN are very high-priced because efficient and facile methods for industrial NMN production are limited. In this study, aiming for its nutraceutical application, we attempted to screen lactic acid bacteria for intracellular and/or extracellular NMN production. Using a bioassay system with an auxotrophic yeast that requires nicotinamide riboside (NR; dephosphorylated NMN), three candidates were obtained from a library of 174 strains of facultative anaerobic lactic acid bacteria. All three candidates belonged to the genus Fructobacillus and produced NR in the culture media (0.8–1.5 mg/l). Lactic acid bacteria of the genus Fructobacillus are known to use d-fructose as an electron acceptor in anaerobic lactic acid fermentation; addition of d-fructose to the medium caused intracellular accumulation of NMN and NR, but no extracellular production of these compounds was observed. Draft genome sequencing for one of the candidates suggested that nicotinamide phosphoribosyltransferase, which exists commonly in mammals but is less reported in microorganisms, is a key enzyme for NMN and NR production in the fructophilic bacteria. |
| Databáze: | OpenAIRE |
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