Identification of conditionally essential genes for Streptococcus suis infection in pigs
| Autor: | Arenas, Jesús, Zomer, Aldert, Harders-Westerveen, Jose, Bootsma, Hester J, De Jonge, Marien I, Stockhofe-Zurwieden, Norbert, Smith, Hilde E, De Greeff, Astrid, Klinische infectiologie en microb. lab., dI&I I&I-4 |
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| Přispěvatelé: | Klinische infectiologie en microb. lab., dI&I I&I-4 |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
transposon mutagenesis Streptococcus suis Immunology Mutant lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Tn-Seq Virulence Infectious and parasitic diseases RC109-216 Microbiology Genome Sepsis 03 medical and health sciences Immune system medicine Gene 030304 developmental biology Host Pathogen Interaction & Diagnostics 0303 health sciences biology 030306 microbiology pathogenesis Bacteriologie Wild type streptococcus suis zoonotic pathogen Bacteriology Bacteriology Host Pathogen Interaction & Diagnostics medicine.disease biology.organism_classification Host Pathogen Interactie & Diagnostiek infection Infectious Diseases Bacteriologie Host Pathogen Interactie & Diagnostiek tn-seq Parasitology |
| Zdroj: | Virulence, 11, 446-464 Virulence, Vol 11, Iss 1, Pp 446-464 (2020) Virulence, 11(1), 446. Landes Bioscience Virulence 11 (2020) 1 Virulence Virulence, 11(1), 446-464 Virulence, 11, 1, pp. 446-464 Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Zaguán: Repositorio Digital de la Universidad de Zaragoza Universidad de Zaragoza |
| ISSN: | 2150-5594 |
| Popis: | Streptococcus suis is a Gram-positive bacterium and zoonotic pathogen that causes meningitis and sepsis in pigs and humans. The aim of this study was to identify genes required for S. suis infection. We created Tn-Seq libraries in a virulent S. suis strain 10, which was used to inoculate pigs in an intrathecal experimental infection. Comparative analysis of the relative abundance of mutants recovered from different sites of infection (blood, cerebrospinal fluid, and meninges of the brain) identified 361 conditionally essential genes, i.e. required for infection, which is about 18% of the genome. The conditionally essential genes were primarily involved in metabolic and transport processes, regulation, ribosomal structure and biogenesis, transcription, and cell wall membrane and envelope biogenesis, stress defenses, and immune evasion. Directed mutants were created in a set of 10 genes of different genetic ontologies and their role was determined in ex vivo models. Mutants showed different levels of sensitivity to survival in whole blood, serum, cerebrospinal fluid, thermic shock, and stress conditions, as compared to the wild type. Additionally, the role of three selected mutants was validated in co-infection experiments in which pigs were infected with both wild type and isogenic mutant strains. The genetic determinants of infection identified in this work contribute to novel insights in S. suis pathogenesis and could serve as targets for novel vaccines or antimicrobial drugs. |
| Databáze: | OpenAIRE |
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