The recombinant EHV-1 vector producing CDV hemagglutinin as potential vaccine against canine distemper
| Autor: | Zihao Pan, Huochun Yao, Qiuli Jin, Nikolaus Osterrieder, Jin Liu, Jiale Ma |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal diseases viruses Genetic Vectors 030106 microbiology Hemagglutinin (influenza) Antibodies Viral Recombinant virus Microbiology Virus Viral vector Viral Proteins 03 medical and health sciences Dogs Morbillivirus medicine Animals Distemper Distemper Virus Canine biology Canine distemper Viral Vaccines biology.organism_classification medicine.disease Virology Nucleoprotein Vaccination Hemagglutinins 030104 developmental biology Infectious Diseases biology.protein Herpesvirus 1 Equid |
| Zdroj: | Microbial Pathogenesis. 111:388-394 |
| ISSN: | 0882-4010 |
| Popis: | Canine distemper virus (CDV), is a pantropic agent of morbillivirus that causes fetal disease in dogs. Base on a broad host rang of CDV, the continued vaccines inoculation is unavoidable to pose gene recombination risk in vaccine virus and wild virus. The current study presents the construction of novel vectors, using equine herpesvirus type 1 (EHV-1) expressing the canine distemper virus (CDV). The recent field strain hemagglutinin protein and nucleoprotein were used for the construction of the viral vector vaccines. Based on the Bacterial artificial chromosome (BAC) genomes of EHV-1 RacH strain, the recombinant EHV-1 vaccine virus encoding CDV hemagglutinin protein (EHV-H) or CDV nucleoprotein (EHV-N) was constructed separately. The constructed BACs were rescued after 72 h post infection, and the expression of H or N in the recombinant viruses was confirmed by western-blotting. Furthermore, high levels of neutralizing antibodies were induced persistently following vaccination in the groups EHV-H&EHV-N and EHV-H, but the EHV-N group. The groups of vaccinated EHV-H and EHV-H&EHV-N pups were monitored for clinical signs, whereas the vaccinated EHV-N group developed moderate symptoms. The present study demonstrated that EHV-1 based recombinant virus carrying CDV H could be a promising vaccine candidate against canine distemper. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect