Binding of histone H1e-c variants to CpG-rich DNA correlates with the inhibitory effect on enzymic DNA methylation
| Autor: | S Marenzi, Roberto Strom, H P Saluz, Paola Caiafa, Anna Reale, Raffaella Santoro, Maria D'Erme, Stefania Mastrantonio |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
histone H1 variants DNA methylation CpG islands Biology Biochemistry Methylation Histones Epigenetics of physical exercise Histone methylation Animals Cancer epigenetics Molecular Biology RNA-Directed DNA Methylation DNA Modification Methylases Epigenomics Binding Sites Base Sequence Genetic Variation Cell Biology DNA Molecular biology CpG site Cattle Dinucleoside Phosphates Research Article |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 0264-6021 |
| Popis: | Within the H1 histone family, only some fractions enriched in the H1e-c variants are effective in causing a marked inhibition, in vitro, of enzymic DNA methylation and, in gel retardation and Southwestern blot experiments, in binding double-stranded (ds) CpG-rich oligonucleotides. Both the 6-CpG ds-oligonucleotide and the DNA purified from chromatin fractions enriched in ‘CpG islands’ are good competitors for the binding of H1e-c to 6-meCpG ds-oligonucleotide. Because of their ability to bind any DNA sequence and to suppress the enzymic methylation in any sequence containing CpG dinucleotides, these particular H1 variants could play some role in maintaining linker DNA at low methylation levels and even in preserving the unmethylated state of the CpG-rich islands which characterize the promoter regions of housekeeping genes. |
| Databáze: | OpenAIRE |
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