Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ
| Autor: | Anna A. Kim, Amanda Nguyen, Marco Marchetti, XinXin Du, Denise J. Montell, Beth L. Pruitt, Lucy Erin O'Brien |
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| Rok vydání: | 2022 |
| Předmět: |
Stem cell
Cell- och molekylärbiologi Enteroendocrine Cells 1.1 Normal biological development and functioning Cell Differentiation Cell Biology Biological Sciences Stem Cell Research Regenerative Medicine Medical and Health Sciences Epithelial cell Midgut Underpinning research Animals Drosophila Proteins Drosophila Calcium Stem Cell Research - Nonembryonic - Non-Human Generic health relevance Cell and Molecular Biology Live imaging Developmental Biology |
| Zdroj: | Journal of cell science, vol 135, iss 14 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.260249 |
| Popis: | Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. Ca2+ dynamics have been studied widely in cellular monocultures, yet organs in vivo comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging of whole organs ex vivo reveals that stem-cell daughters adopt strikingly distinct patterns of Ca2+ oscillations after differentiation: enteroendocrine cells exhibit single-cell Ca2+ oscillations, whereas enterocytes exhibit rhythmic, long-range Ca2+ waves. These multicellular waves do not propagate through immature progenitors (stem cells and enteroblasts), of which the oscillation frequency is approximately half that of enteroendocrine cells. Organ-scale inhibition of gap junctions eliminates Ca2+ oscillations in all cell types – even, intriguingly, in progenitor and enteroendocrine cells that are surrounded only by enterocytes. Our findings establish that cells adopt fate-specific modes of Ca2+ dynamics as they terminally differentiate and reveal that the oscillatory dynamics of different cell types in a single, coherent epithelium are paced independently. |
| Databáze: | OpenAIRE |
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