The csnD/csnE Signalosome Genes Are Involved in the Aspergillus nidulans DNA Damage Response
| Autor: | Marcia Regina von Zeska Kress Fagundes, Iran Malavazi, Gustavo H. Goldman, Maria Helena S. Goldman, Marcela Savoldi, Gerhard H. Braus, Elke U. Schwier, Joel Fernandes Lima |
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| Rok vydání: | 2005 |
| Předmět: |
DNA Repair
DNA damage DNA repair Genes Fungal Mutant Investigations Aspergillus nidulans 03 medical and health sciences chemistry.chemical_compound Ribonucleotide Reductases Genetics RNA Messenger Gene 030304 developmental biology 0303 health sciences DNA synthesis biology COP9 Signalosome Complex Reproduction 030302 biochemistry & molecular biology biology.organism_classification Molecular biology Methyl methanesulfonate Ribonucleotide reductase chemistry Multiprotein Complexes DNA Damage Peptide Hydrolases |
| Zdroj: | Genetics. 171:1003-1015 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.105.041376 |
| Popis: | The signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development and is also required to activate ribonucleotide reductase for DNA synthesis. In Aspergillus nidulans, csnD/csnE are key regulators of sexual development. Here, we investigated whether the csnD/csnE genes are involved in the DNA damage response in this fungus. The growth of the csnD/csnE deletion mutants was reduced by subinhibitory concentrations of hydroxyurea, camptothecin, 4-nitroquinoline oxide, and methyl methanesulfonate. A. nidulans increases csnD/csnE mRNA levels when it is challenged by different DNA-damaging agents. There is no significant transcriptional induction of the csnE promoter fused with lacZ gene in the presence of DNA-damaging agents, suggesting that increased mRNA accumulation is due to increased mRNA stability. Septation was not inhibited in the csnD/csnE deletion mutants while ΔuvsB ΔcsnE presented an increase in septation upon DNA damage caused by methyl methanesulfonate, suggesting that uvsBATR and csnE genetically interact during checkpoint-dependent inhibition of septum formation. The double ΔcsnD/ΔcsnE ΔnpkA mutants were more sensitive to DNA-damaging agents than were the respective single mutants. Our results suggest that csnD/csnE genes are involved in the DNA damage response and that NpkA and UvsBATR genetically interact with the signalosome. |
| Databáze: | OpenAIRE |
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