GM-CSF and GM-CSF receptor have regulatory role in transforming rat mesenteric mesothelial cells into macrophage-like cells
| Autor: | Viktória Zsiros, Arnold Szabo, Nikolett Dóczi, Sándor Katz, Anna L. Kiss, Adam Biczo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty media_common.quotation_subject Immunology Cell Freund's Adjuvant Inflammation Peritonitis Epithelium Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo medicine Macrophage Animals Internalization Autocrine signalling Cells Cultured media_common Pharmacology Chemistry Granulocyte-Macrophage Colony-Stimulating Factor Epithelial Cells Molecular biology In vitro 030104 developmental biology medicine.anatomical_structure Receptors Granulocyte-Macrophage Colony-Stimulating Factor Macrophages Peritoneal medicine.symptom Mesothelial Cell 030215 immunology |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 65(10) |
| ISSN: | 1420-908X |
| Popis: | During peritonitis, mesothelial cells assume macrophage characteristics, expressing macrophage markers, indicating that they might differentiate into macrophage-like cells. Twenty-five male rats were used for in vivo experiments. For in vitro experiments, a primary mesentery culture model was developed. The mesothelial cell to macrophage-like cell transition was followed by studying ED1 expression. In vitro primary mesenteric culture was treated with granulocyte–macrophage colony-stimulating factor (GM-CSF, 1 ng/ml). Blocking internalization of receptor–ligand complex, Dynasore (80 µM) was used. Acute peritonitis was induced by Freund’s adjuvant’s (1 ml) intraperitoneal injection. Immunohistochemistry: GM-CSF in vitro treatment resulted in a prominent ED1 expression in transformed mesothelial cells. Blocking the internalization, ED1 expression could not be detected. GM-CSF receptor (both α and β) was expressed in mesothelial cells in vitro (even if the GM-CSF was not present) and in vivo. Inflammation resulted in an increasing GM-CSF and GM-CSF-receptor level in the lysate of mesothelial cells. Mesothelial cells can differentiate into macrophage-like cells, and GM-CSF, produced by the mesothelial cells, has probably an autocrine regulatory role in this transition. Our results provide new data about the plasticity of mesothelial cell and support the idea that during inflammation macrophages can derive from non-hematopoietic sources as well. |
| Databáze: | OpenAIRE |
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