Identification of Hsp90 as a Stimulatory Host Factor Involved in Influenza Virus RNA Synthesis
| Autor: | Tadasuke Naito, Fumitaka Momose, Keiichi Yano, Kyosuke Nagata, Seiji Sugimoto, Yuko Morikawa |
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| Rok vydání: | 2002 |
| Předmět: |
Gene Expression Regulation
Viral Recombinant Fusion Proteins viruses RNA-dependent RNA polymerase Biochemistry Virus Cell Line Viral Proteins Transcription (biology) Sigma factor Viral entry RNA polymerase I Humans HSP70 Heat-Shock Proteins Amino Acid Sequence HSP90 Heat-Shock Proteins Molecular Biology Polymerase Cell Nucleus Binding Sites biology RNA DNA-Directed RNA Polymerases Cell Biology RNA-Dependent RNA Polymerase Virology Molecular biology Protein Structure Tertiary Protein Subunits Influenza A virus biology.protein RNA Viral Molecular Chaperones Protein Binding |
| Zdroj: | Journal of Biological Chemistry. 277:45306-45314 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m206822200 |
| Popis: | Efficient transcription and replication of the influenza virus genome are dependent upon host-derived factors. Using an in vitro RNA synthesis system, we have purified and identified Hsp90 as one of the host factors that stimulate viral RNA polymerase activity. Hsp90 interacted with the PB2 subunit of the viral RNA polymerase through the amino-terminal chaperone domain and the middle region containing a highly acidic domain. The acidic middle region was also responsible for its stimulatory activity. We found that a portion of Hsp90 is re-localized to the cell nucleus after viral infection. A PB2 fragment containing a Hsp90 binding domain inhibited viral gene expression in a dominant-negative manner. These results suggest that Hsp90 is a host factor for the influenza virus RNA polymerase. |
| Databáze: | OpenAIRE |
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