Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach

Autor:	Leichtfried, Thomas, Reisenzein, Helga, Steinkellner, Siegrid, Gottsberger, Richard A.
Rok vydání:	2020
Předmět:	0106 biological sciences 0301 basic medicine 03 medical and health sciences 030104 developmental biology Brief Report Virology General Medicine 01 natural sciences 010606 plant biology & botany
Zdroj:	Archives of Virology
ISSN:	1432-8798 0304-8608
DOI:	10.1007/s00705-020-04704-5
Popis:	The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids (Myzus persicae, Dysaphis plantaginea) and in codling moths (Cydia pomonella). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b76b362240e18f6686e44725285fcebd https://doi.org/10.1007/s00705-020-04704-5 Zobrazit plný text záznamu Full text from SpringerLink