H3.3 demarcates GC-rich coding and subtelomeric regions and serves as potential memory mark for virulence gene expression in Plasmodium falciparum
| Autor: | Fraschka, Sabine Anne-Kristin, Henderson, Rob Wilhelmus Maria, Bártfai, Richárd |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation Plasmodium falciparum Protozoan Proteins Article Histones 03 medical and health sciences Histone H3 Histone H1 Histone code Promoter Regions Genetic GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Gene Molecular Biology Gene Library Histone variants Genetics Life Cycle Stages Multidisciplinary Virulence biology Sequence Analysis RNA Parasite genomics High-Throughput Nucleotide Sequencing DNA Protozoan Subtelomere Chromatin GC Rich Sequence 030104 developmental biology Histone biology.protein Genome Protozoan Chromatin immunoprecipitation RNA Protozoan |
| Zdroj: | Scientific Reports, 6, pp. 1-14 Scientific Reports Scientific Reports, 6, 1-14 |
| ISSN: | 2045-2322 |
| Popis: | Histones, by packaging and organizing the DNA into chromatin, serve as essential building blocks for eukaryotic life. The basic structure of the chromatin is established by four canonical histones (H2A, H2B, H3 and H4), while histone variants are more commonly utilized to alter the properties of specific chromatin domains. H3.3, a variant of histone H3, was found to have diverse localization patterns and functions across species but has been rather poorly studied in protists. Here we present the first genome-wide analysis of H3.3 in the malaria-causing, apicomplexan parasite, P. falciparum, which revealed a complex occupancy profile consisting of conserved and parasite-specific features. In contrast to other histone variants, PfH3.3 primarily demarcates euchromatic coding and subtelomeric repetitive sequences. Stable occupancy of PfH3.3 in these regions is largely uncoupled from the transcriptional activity and appears to be primarily dependent on the GC-content of the underlying DNA. Importantly, PfH3.3 specifically marks the promoter region of an active and poised, but not inactive antigenic variation (var) gene, thereby potentially contributing to immune evasion. Collectively, our data suggest that PfH3.3, together with other histone variants, indexes the P. falciparum genome to functionally distinct domains and contribute to a key survival strategy of this deadly pathogen. |
| Databáze: | OpenAIRE |
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