Tranilast inhibits the proliferation of uterine leiomyoma cells in vitro through G1 arrest associated with the induction of p21(waf1) and p53
| Autor: | Takashi Kusakari, Kenji Takakura, Hiroaki Shime, Toshio Nikaido, Shingo Fujii, Masatoshi Kariya, Takanobu Kanamori, Chika Momma, Ken Fukuhara, Ayaka Orii, Yuko Tsuruta |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
medicine.medical_specialty Vascular smooth muscle Endocrinology Diabetes and Metabolism Tranilast Clinical Biochemistry Blotting Western Biology Protein Serine-Threonine Kinases Biochemistry Endocrinology Internal medicine Cyclins medicine CDC2-CDC28 Kinases Tumor Cells Cultured Cytotoxic T cell Humans ortho-Aminobenzoates neoplasms Uterine leiomyoma Leiomyoma Cell growth Biochemistry (medical) Cell Cycle Cyclin-Dependent Kinase 2 Myometrium G1 Phase Muscle Smooth medicine.disease Genes p53 female genital diseases and pregnancy complications Cyclin-Dependent Kinases Gene Expression Regulation Cell culture Uterine Neoplasms Female Cell Division medicine.drug |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 87(12) |
| ISSN: | 0021-972X |
| Popis: | Uterine leiomyoma is a mesenchymal tumor composed of smooth muscle cells with fibrous tissues and many mast cells. Tranilast is known to suppress fibrosis or to work as a mast cell stabilizer and is reported to inhibit proliferation of vascular smooth muscle cells. In this study, we examined the effects of tranilast on cultured human leiomyoma cells in vitro to evaluate whether this agent has the potential to inhibit the growth of uterine leiomyomas. Tranilast inhibited the proliferation of cultured leiomyoma cells in a dose-dependent manner without any cytotoxic effect or induction of apoptosis. In association with the inhibitory effect, tranilast induced the cyclin-dependent kinase (CDK) inhibitor p21(waf1) and tumor suppressor gene p53 and decreased CDK2 activity. These results suggest that tranilast arrests the proliferation of uterine leiomyoma cells at the G0/G1 phase, through the suppression of CDK2 activity via an induction of p21(waf1) and p53. Tranilast was concluded to be a potent agent to inhibit proliferative activity of uterine leiomyoma cells. |
| Databáze: | OpenAIRE |
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