No primary association of MICA polymorphism with systemic lupus erythematosus
| Autor: | M F González-Escribano, Elena Sánchez, Miguel A. González-Gay, Miguel A. López-Nevot, Javier Martín, B. Torres, Antonio Núñez-Roldán, E de Ramón, N. Ortego-Centeno, J. R. Vilches, J. Sánchez-Román, J. Jiménez-Alonso |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty Linkage disequilibrium Adolescent Genotype Gastroenterology Gene Frequency Rheumatology Immunopathology Internal medicine Humans Lupus Erythematosus Systemic Medicine Genetic Predisposition to Disease Pharmacology (medical) Genotyping HLA-DRB1 Chi-Square Distribution Polymorphism Genetic business.industry Histocompatibility Antigens Class I HLA-DR Antigens Odds ratio Middle Aged Confidence interval HLA-B HLA-B Antigens Case-Control Studies Immunology Female Gene polymorphism business HLA-DRB1 Chains |
| Zdroj: | Rheumatology. 45:1096-1100 |
| ISSN: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/kel058 |
| Popis: | OBJECTIVE To replicate the described association between MHC class I chain-related A (MICA) gene polymorphism and susceptibility to systemic lupus erythematosus (SLE). METHODS MICA transmembrane microsatellite polymorphism was genotyped using a polymerase chain reaction (PCR)-based method. Genotyping of HLA-B* and DRB1* was performed using PCR and detection with a reverse sequence-specific oligonucleotide (SSO) probe system. Combined data for these three loci (HLA-B*, DRB1* and MICA) were obtained from a total of 333 patients and 361 healthy controls. RESULTS Significant association with B*08 [P < 10(-7), odds ratio (OR) 3.17, 95% confidence interval (CI) 2.02-5.00], DRB1*0301 (P < 10(-7), OR 2.07, 95% CI 1.59-2.68) and MICA5.1 (P = 0.01, OR 1.23, 95% CI 1.04-1.46) was observed. The combinations DRB1*0301-MICA5.1-B8 and HLA-DRB1*0301-B*08-positive and MICA5-1-negative were more frequent among SLE patients (11.4 vs 3.3% in healthy controls, P = 3.9 x 10(-5), OR 3.76, 95% CI 1.85-7.73, and 6.9 vs 1.7%, P = 0.0007, OR 4.32, 95% CI 1.68-13.10, respectively). Additionally, individuals who were HLA-DRB1*0301-B*08-negative and MICA5-1-positive were less frequent among patients (22.2 vs 31.3% in healthy controls, P = 0.007, OR 0.63, 95% CI 0.44-0.89) and the magnitude of the OR was similar to that obtained in individuals negative for all the three factors (OR 0.69, 95% CI 050-0.94). Further analysis performed to detect independent association strongly suggested that the association between MICA5.1 and SLE is secondary to the linkage disequilibrium of this allele with B*08. CONCLUSIONS Our results do not support an independent association of MICA gene polymorphism with susceptibility to SLE. |
| Databáze: | OpenAIRE |
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