Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats

Autor: Dun Xian Tan, Aroa Jiménez-Aranda, Ahmad Agil, Russel J. Reiter, Lourdes Velasco-Perez, Mohamed Tassi, Daniel Campos, Gumersindo Fernández-Vázquez
Rok vydání: 2013
Předmět:
Zdroj: Journal of Pineal Research. 55:416-423
ISSN: 0742-3098
DOI: 10.1111/jpi.12089
Popis: Melatonin limits obesity in rodents without affecting food intake and activity, suggesting a thermogenic effect. Identification of brown fat (beige/brite) in white adipose tissue (WAT) prompted us to investigate whether melatonin is a brown-fat inducer. We used Zucker diabetic fatty (ZDF) rats, a model of obesity-related type 2 diabetes and a strain in which melatonin reduces obesity and improves their metabolic profiles. At 5 wk of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control and those treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 wk. Melatonin induced browning of inguinal WAT in both ZDF and ZL rats. Hematoxylin-eosin staining showed patches of brown-like adipocytes in inguinal WAT in ZDF rats and also increased the amounts in ZL animals. Inguinal skin temperature was similar in untreated lean and obese rats. Melatonin increased inguinal temperature by 1.36 ± 0.02°C in ZL and by 0.55 ± 0.04°C in ZDF rats and sensitized the thermogenic effect of acute cold exposure in both groups. Melatonin increased the amounts of thermogenic proteins, uncoupling protein 1 (UCP1) (by ~2-fold, P
Databáze: OpenAIRE
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