Catalysis of nitrosyl transfer by denitrifying bacteria is facilitated by nitric oxide
| Autor: | Thomas C. Hollocher, Joanne Goretski |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Azides
Denitrification Nitric-oxide reductase Nitrous Oxide Biophysics Nitric Oxide Photochemistry Biochemistry Catalysis Nitric oxide Denitrifying bacteria chemistry.chemical_compound Pseudomonas Alcaligenes Nitrite Sodium Azide Molecular Biology Nitrogen Isotopes Sodium Nitrite biology Chemistry Cell Biology biology.organism_classification Nitrite reductase Pseudomonas stutzeri Azide |
| Zdroj: | Biochemical and Biophysical Research Communications. 175:901-905 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(91)91650-2 |
| Popis: | Two denitrifying bacteria, Pseudomonas stutzeri and Achromobacter cycloclastes, were incubated with Na15NO2 and NaN3 under conditions that allowed catalysis of nitrosyl transfer from nitrite to azide. This transfer, which is presumed to be mediated by the heme- and copper-containing nitrite reductase of P. stutzeri and A. cycloclastes, respectively, leads to formation of isotopically mixed 14,15N2O, whereas denitrification leads to 15N2O. The conditions that emphasized nitrosyl transfer also partially inhibited the nitric oxide reductase system and led to accumulation of 15NO. Absorption of NO from the gas phase by acidic CrSO4 in a sidewell largely abolished nitrosyl transfer to azide. With these two organisms, which are thought to be representative of denitrifiers generally, catalysis of nitrosyl transfer seemed to depend on NO. |
| Databáze: | OpenAIRE |
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