Mutagenesis of Saccharomyces cerevisiae by sodium azide activated in barley
| Autor: | J. Velemínský, T. Gichner, L. Šilhánková, V. Smiovská |
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| Rok vydání: | 1979 |
| Předmět: |
Azides
Health Toxicology and Mutagenesis Saccharomyces cerevisiae Mutant Locus (genetics) Cycloheximide chemistry.chemical_compound Genetics Mutation frequency Molecular Biology biology food and beverages Drug Resistance Microbial Hordeum biology.organism_classification Phenotype chemistry Biochemistry Mutation Sodium azide Isoleucine Ploidy Edible Grain Mutagens |
| Zdroj: | Mutation research. 61(2) |
| ISSN: | 0027-5107 |
| Popis: | Concentrated dialysate of the extract prepared from barley seeds treated with sodium azide increased up to 100–200 times the frequency of forward mutations to cycloheximide resistance in the excision-deficient UV-sensitive haploid strain rad2–5 of Saccharomyces cerevisiae , when applied to growing cells in complete medium at pH 4.2. Only a slight increase of mutation frequency (less than 4 times) was found in the haploid RAD + strain treated in the same way as well as in haploid RAD + and rad2-5 strains treated directly by sodium azide. In contrast with the barley-activated sodium azide, UV irradiation was more effective in the induction of cycloheximide resistance in the RAD + strain than in the rad2-5 mutant. The dialysate from azide-treated barley seeds, applied at both pH 4.2 and pH 9, also significantly increased the frequency of locus-specific suppressor mutations to isoleucine independence and — to a lesser extent — reversions and/or gene conversions in the trp5 locus in growing cells of the diploid strain D7. The dialysate was also mutagenic in resting cells of strains D7 and rad2-5 but with lower effectiveness. |
| Databáze: | OpenAIRE |
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