α-Linolenic Acid Intake Attenuates Myocardial Ischemia/Reperfusion Injury through Anti-inflammatory and Anti-oxidative Stress Effects in Diabetic But Not Normal Rats

Autor: Xuezeng Xu, Haifeng Zhang, Hongliang Liang, Weixun Duan, Jia Li, Huasong Zhou, Shiqiang Yu, Wei Zhang, Nianlin Xie, Dinghua Yi
Rok vydání: 2011
Předmět:
Blood Glucose
Male
Anti-Inflammatory Agents
medicine.disease_cause
Antioxidants
Rats
Sprague-Dawley
Phosphatidylinositol 3-Kinases
Superoxides
Malondialdehyde
Insulin
Myocardial infarction
Phosphorylation
Creatine Kinase
Cardioprotection
Membrane Glycoproteins
biology
alpha-Linolenic Acid
Heart
General Medicine
Anesthesia
NADPH Oxidase 2
medicine.drug
medicine.medical_specialty
Normal diet
Ischemia
Myocardial Reperfusion Injury
Diabetes Mellitus
Experimental
Necrosis
Internal medicine
medicine
Animals
Triglycerides
L-Lactate Dehydrogenase
Superoxide Dismutase
business.industry
Myocardium
Body Weight
NADPH Oxidases
Recovery of Function
Streptozotocin
medicine.disease
Dietary Fats
Rats
Oxidative Stress
Endocrinology
Dietary Supplements
biology.protein
Creatine kinase
business
Proto-Oncogene Proteins c-akt
Reperfusion injury
Oxidative stress
Zdroj: Archives of Medical Research. 42:171-181
ISSN: 0188-4409
Popis: Background and Aims Patients with diabetes show enhanced susceptibility to myocardial ischemia/reperfusion (MI/R) injury. Epidemiological studies indicated that consumption of α-linolenic acid (ALA) significantly reduces the risk of cardiac events in post-acute myocardial infarction patients. The present study attempted to investigate the effects of ALA intake on MI/R injury in normal and diabetic rats and its mechanisms. Methods The high-fat diet-fed streptozotocin (HFD-STZ) rat model was developed. Age-matched normal and HFD-STZ rats were randomly assigned to receive normal diet or ALA (oral gavage, 500 μg/kg per day). After 4 weeks of feeding, animals were subjected to 30 min of myocardial ischemia and 4 or 6 h of reperfusion. Results Compared with the normal control, HFD-STZ rats showed more severe myocardial functional impairment and injury. Although ALA intake for 4 weeks did not change myocardial function and injury in normal rats, it significantly improved the instantaneous first derivation of left ventricle pressure, reduced infarct size, plasma creatine kinase and lactate dehydrogenase activities, and apotosis at the end of reperfusion in HFD-STZ diabetic rats. Moreover, ALA intake not only significantly reduced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations but reduced the increase in superoxide production and malonaldialdehyde formation and simultaneously enhanced the antioxidant capacity in the diabetic hearts. Myocardial PI3K expression and Akt phosphorylation were increased by ALA intake in diabetic but not normal rats. Conclusions Chronic ALA intake confers cardioprotection in MI/R by exerting anti-inflammatory and anti-oxidative stress effects in diabetic but not normal rats, which is possibly through PI3K-Akt-dependent mechanism.
Databáze: OpenAIRE
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