Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis
| Autor: | Xiaodong Zhang, Daniela A Parodi, Martin Dym, Yixun Geng, Carlos A. Suárez-Quian, Zuping He, Lixin Feng |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system Embryology medicine.medical_specialty Blotting Western macromolecular substances Testicle Biology Collagen Type I Andrology Mice Endocrinology Western blot Meiosis Internal medicine medicine Animals Spermatogenesis Oligonucleotide Array Sequence Analysis Mice Inbred BALB C integumentary system medicine.diagnostic_test urogenital system Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Cell Biology Seminiferous Tubules Sertoli cell Spermatogonia Blot Collagen Type I alpha 1 Chain medicine.anatomical_structure Reproductive Medicine Immunohistochemistry Collagen Germ cell |
| Zdroj: | Reproduction (Cambridge, England). 130(3) |
| ISSN: | 1470-1626 |
| Popis: | The objective of this study was to compare the expression of Col1a1, Col1a2, and procollagen I in the seminiferous tubules of immature and adult mice and to characterize the cellular expression pattern of procollagen I in germ cells during spermatogenesis in order to provide necessary groundwork for further functional studies in the process of spermatogenesis. Microarray analysis demonstrated that Col1a1 and Col1a2 were abundantly expressed in the seminiferous tubules of 6-day-old mice compared with 60-day-old mice, and the expression levels of Col1a1 and Col1a2 mRNA were validated using a semi-quantitative RT-PCR assay. Western blot analysis further confirmed that procollagen I was expressed at a higher level in the seminiferous tubules of 6-day-old mice compared with 60-day-old mice. Immunohistochemical analysis revealed that type A spermatogonia were positive for procollagen I in the testis of 6-day-old mice, whereas Sertoli cells were negative for this protein. Thein vivoprocollagen I staining in type A spermatogonia was corroborated in spermatogonia exhibiting a high potential for proliferation and the ability to form germ cell colonies inin vitroculture. Moreover, procollagen I was also detected in type A spermatogonia, intermediate spermatogonia, type B spermatogonia, and preleptotene spermatocytes in the adult mouse testes, but positive staining disappeared in more differentiated germ cell lineages detaching from the basement membrane, including leptotene spermatocytes, pachytene spermatocytes, round spermatids and elongated spermatids. These data suggest that Col1a1, Col1a2 and procollagen I are associated with type A spermatogonia and play a potential role in mediating the detachment and migration of germ cells during spermatogenesis. |
| Databáze: | OpenAIRE |
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