Humoral response in experimental autoimmune encephalomyelitis targets neural precursor cells in the central nervous system of naive rodents

Autor: Olga Touloumi, Constantina Simeonidou, Georgios M. Hadjigeorgiou, Evangelia Kesidou, Nickoleta Delivanoglou, Kyriaki-Nepheli Poulatsidou, Roza Lagoudaki, Evangelia Nousiopoulou, Nikolaos Grigoriadis, Fani Minti, Evangelia Kofidou, Marina Boziki, Paschalis Theotokis
Rok vydání: 2017
Předmět:
0301 basic medicine
Cell type
Doublecortin Protein
Encephalomyelitis
Autoimmune
Experimental
Subventricular zone
Immunology
Neural precursor cells
Apoptosis
Autoantigens
lcsh:RC346-429
Myelin oligodendrocyte glycoprotein
03 medical and health sciences
Cellular and Molecular Neuroscience
Mice
0302 clinical medicine
Autoantibody
Neural Stem Cells
Precursor cell
Lateral Ventricles
medicine
otorhinolaryngologic diseases
Animals
Remyelination
lcsh:Neurology. Diseases of the nervous system
Autoantibodies
Experimental autoimmune encephalomyelitis
biology
General Neuroscience
Research
Colocalization
Nestin
medicine.disease
Peptide Fragments
Cell biology
Immunity
Humoral
Mice
Inbred C57BL
stomatognathic diseases
030104 developmental biology
medicine.anatomical_structure
Neurology
biology.protein
Female
Myelin-Oligodendrocyte Glycoprotein
030217 neurology & neurosurgery
Zdroj: Journal of Neuroinflammation
Journal of Neuroinflammation, Vol 14, Iss 1, Pp 1-14 (2017)
ISSN: 1742-2094
Popis: Background Neural precursor cells (NPCs) located in the subventricular zone (SVZ), a well-defined NPC niche, play a crucial role in central nervous system (CNS) homeostasis. Moreover, NPCs are involved in the endogenous reparative process both in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). However, the possibility that NPCs may be vulnerable to immune-related components may not be ruled out. Therefore, we investigated the potential affinity of myelin oligodendrocyte glycoprotein (MOG)-induced humoral response(s) to NPCs. Methods MOG35–55-EAE was induced in C57BL/6 mice; blood-sampling was performed on days 17–21 (acute phase) along with a naive group and corresponding antisera (AS) were collected (EAE-AS, NAIVE-AS). The presence of anti-CNS autoantibodies was examined with western blotting. Furthermore, using the collected antisera and anti-MOG antibody (as positive control), immunohistochemistry and double immunofluorescence were implemented on normal neonatal, postnatal, and adult mouse brain sections. Targeted NPCs were identified with confocal microscopy. In vitro immunoreactivity assessment on NPCs challenged with autoantibodies was evaluated for apoptotic/autophagic activity. Results Western blotting verified the existence of autoantibodies in EAE mice and demonstrated bands corresponding to yet unidentified NPC surface epitopes. A dominant selective binding of EAE-AS in the subventricular zone in all age groups compared to NAIVE-AS (p
Databáze: OpenAIRE
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