Nanoparticle–Cell Interactions: Surface Chemistry Effects on the Cellular Uptake of Biocompatible Block Copolymer Assemblies
| Autor: | František Surman, Lindomar J. C. Albuquerque, Cristiano Giacomelli, Fernando C. Giacomelli, Caroline Ribeiro, Maria Cristina Carlan da Silva, Alex Carvalho Alavarse, Vanessa Schmidt, Eliézer Jäger, Carlos E. de Castro |
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| Rok vydání: | 2018 |
| Předmět: |
Polymers
Surface Properties Nanoparticle Biocompatible Materials 02 engineering and technology 010402 general chemistry Methacrylate Hemolysis 01 natural sciences Turn (biochemistry) chemistry.chemical_compound Electrochemistry Copolymer Humans Methacrylamide General Materials Science Spectroscopy Ethylene oxide Phosphorylcholine Chemistry Biological Transport Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Chemical engineering Nanoparticles 0210 nano-technology Layer (electronics) HeLa Cells |
| Zdroj: | Langmuir. 34:2180-2188 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | The development of nanovehicles for intracellular drug delivery is strongly bound to the understating and control of nanoparticles cellular uptake process, which in turn is governed by surface chemistry. In this study, we explored the synthesis, characterization, and cellular uptake of block copolymer assemblies consisting of a pH-responsive poly[2-(diisopropylamino)ethyl methacrylate] (PDPA) core stabilized by three different biocompatible hydrophilic shells (a zwitterionic type poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) layer, a highly hydrated poly(ethylene oxide) (PEO) layer with stealth effect, and an also proven nontoxic and nonimmunogenic poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) layer). All particles had a spherical core–shell structure. The largest particles with the thickest hydrophilic stabilizing shell obtained from PMPC40-b-PDPA70 were internalized to a higher level than those smaller in size and stabilized by PEO or PHPMA and produced from PEO122-b-PDPA43 or PHPMA64-b-PDPA72... |
| Databáze: | OpenAIRE |
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