Characterisation of Apoptosis in myb-Transformed Hematopoietic Cell (MTHC-A) Lines : TNF-^|^alpha;-Induced Apoptosis and Prevention by cAMP#
| Autor: | Yuko Kaneko, Paul Waring, Andrew J. Hapel, Ichio Fukasawa, Nobuhide Masawa, Kenji Sorimachi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.drug_class
Genistein Antineoplastic Agents Apoptosis Biology Leukemia Myelomonocytic Acute Tyrosine-kinase inhibitor Mice Proto-Oncogene Proteins c-myb chemistry.chemical_compound Cell Line Tumor Cyclic AMP medicine Animals Tyrosine Protein kinase A Protein Kinase Inhibitors Cell Line Transformed Tumor Necrosis Factor-alpha General Medicine Haematopoiesis Cell Transformation Neoplastic chemistry Cancer research Phosphorylation Tumor necrosis factor alpha |
| Zdroj: | Journal of Clinical and Experimental Hematopathology. 53:115-120 |
| ISSN: | 1880-9952 1346-4280 |
| DOI: | 10.3960/jslrt.53.115 |
| Popis: | 4) Myb-transformed haematopoietic cell (MTHC) lines have been developed and clones selected based on their ability to respond to tumor necrosis factor (TNF)-a. MTHC-A cells underwent apoptosis in response to TNF-a (MTHC-A). The apoptotic effect of TNF-a in MTHC-A was mimicked by a specific inhibitor of protein kinase A (PKI 5-24) and by the tyrosine kinase inhibitor genistein, suggesting that phosphorylation of tyrosine and PKA activity were important in protecting MTHC from apoptosis. Agents that elevate intracellular levels of cAMP, e.g. cholera toxin and dibuteryl cAMP, protected MTHC-A from the apoptotic effects of TNF-a, and also reduced the apoptotic effects of PKI and genistein. MTHC-A thus provides a useful model for investigating the role of TNF-mediated apoptosis in regulation of the myeloid lineage of cells. 〔J Clin Exp Hematop 53(2) : 115-120, 2013〕 |
| Databáze: | OpenAIRE |
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