Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase
| Autor: | Kyoko Tominaga, Kentaro Kogure, Akira Tokumura, Kenji Fukuzawa, Katsuhiko Yasuda, Yuko Kariya, Eiji Majima |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Molecular Sequence Data Biology Biochemistry law.invention Substrate Specificity chemistry.chemical_compound law Multienzyme Complexes Pregnancy Lysophosphatidic acid Animals Humans Amino Acid Sequence Pyrophosphatases Molecular Biology Glycoproteins chemistry.chemical_classification Pyrophosphatase LPAR1 Dose-Response Relationship Drug Nucleotides Phosphoric Diester Hydrolases Glucose-6-Phosphate Isomerase Phosphodiesterase Cell Biology Chromatography Ion Exchange Molecular biology Recombinant Proteins Rats Kinetics Lysophosphatidylcholine Enzyme chemistry Phosphodiesterase I Recombinant DNA Electrophoresis Polyacrylamide Gel Female Autotaxin Lysophospholipids |
| Zdroj: | The Journal of biological chemistry. 277(42) |
| ISSN: | 0021-9258 |
| Popis: | We purified human plasma lysophospholipase D that produces physiologically active lysophosphatidic acid and showed that it is a soluble form of autotaxin, an ecto-nucleotide pyrophosphatase/phosphodiesterase, originally found as a tumor cell motility-stimulating factor. Its lower K(m) value for a lysophosphatidylcholine than that for a synthetic substrate of nucleotide suggests that lysophosphatidylcholine is a more likely physiological substrate for autotaxin and that its predicted physiological and pathophysiological functions could be mediated by its activity to produce lysophosphate acid, an intercellular mediator. Recombinant autotaxin was found to have lysophospholipase D activity; its substrate specificity and metal ion requirement were the same as those of the purified plasma enzyme. The activity of lysophospholipase D for exogenous lysophosphatidylcholine in human serum was found to increase in normal pregnant women at the third trimester of pregnancy and to a higher extent in patients in threatened preterm delivery, suggesting its roles in induction of parturition. |
| Databáze: | OpenAIRE |
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