Ribosomal subunits and ribosomal proteins of Tetrahymena thermophila. Effect of the presence of iodoacetamide during ribosome extraction on the properties of the subunits
| Autor: | Marguerite Cuny, Barbara Petridou, Marie-France Guérin, Françoise Hayes |
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| Přispěvatelé: | Institut de Biologie Physico-Chimique [Institut Curie, Paris] (IBPC), Institut Curie [Paris] |
| Rok vydání: | 1983 |
| Předmět: |
Ribosomal Proteins
Chemical Phenomena Iodoacetates Biochemistry Ribosome Iodoacetamide 03 medical and health sciences chemistry.chemical_compound Ribosomal protein Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology 0303 health sciences biology Molecular mass 030302 biochemistry & molecular biology Tetrahymena Ribosomal RNA biology.organism_classification Molecular Weight Chemistry chemistry Cytoplasm Protozoa Electrophoresis Polyacrylamide Gel Ribosomes |
| Zdroj: | European Journal of Biochemistry European Journal of Biochemistry, 1983, 135 (3), pp.425-434. ⟨10.1111/j.1432-1033.1983.tb07669.x⟩ |
| ISSN: | 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1983.tb07669.x⟩ |
| Popis: | International audience; Proteolytic degradation of ribosomal proteins occurs during the preparation of subunits of the cytoplasmic ribosomes of the protozoa Tetrahymenu thernioplrila and the isolated subunits are inactive. Addition of 5 mM iodoacetamide to cell suspensions before extraction inhibits proteolytic activity and permits isolation of active subunits. The protein complements of these subunits have been characterized in two different two-dimensional elcctrophoretic systems, and their molecular weights have been determined. |
| Databáze: | OpenAIRE |
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