Candida albicans Bgl2p, Ecm33p, and Als1p proteins are involved in adhesion to saliva-coated hydroxyapatite
| Autor: | Richard D. Cannon, Takahiro Oura, Ann R. Holmes, Susumu Kajiwara, Rouyu Zhang, Xinyue Chen, Shun Iwatani, Hoa Thanh Nguyen, Kyoko Niimi, Masakazu Niimi, Naoki Inokawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Saliva cell wall protein C. albicans c. albicans Infectious and parasitic diseases RC109-216 Microbiology 03 medical and health sciences Opportunistic pathogen 0302 clinical medicine parasitic diseases medicine Dentistry (miscellaneous) Candida albicans Cell adhesion saliva biology Chemistry cell adhesion 030206 dentistry Adhesion biology.organism_classification Tooth enamel Yeast QR1-502 stomatognathic diseases tooth enamel 030104 developmental biology Infectious Diseases medicine.anatomical_structure Original Article Research Article |
| Zdroj: | Journal of Oral Microbiology, Vol 13, Iss 1 (2021) Journal of Oral Microbiology article-version (VoR) Version of Record |
| ISSN: | 2000-2297 |
| Popis: | Introduction: Candida albicans is an opportunistic pathogen that causes oral candidiasis. A previous study showed that Bgl2p and Ecm33p may mediate the interaction between the yeast and saliva-coated hydroxyapatite (SHA; a model for the tooth surface). This study investigated the roles of these cell wall proteins in the adherence of C. albicans to SHA beads. Methods: C. albicans BGL2 and ECM33 null mutants were generated from wild-type strain SC5314 by using the SAT1-flipper gene disruption method. A novel method based on labelling the yeast with Nile red, was used to investigate the adherence. Results: Adhesion of bgl2Δ and ecm33Δ null mutants to SHA beads was 76.4% and 64.8% of the wild-type strain, respectively. Interestingly, the adhesion of the bgl2Δ, ecm33Δ double mutant (87.7%) was higher than that of both single mutants. qRT-PCR analysis indicated that the ALS1 gene was over-expressed in the bgl2Δ, ecm33Δ strain. The triple null mutant showed a significantly reduced adherence to the beads, (37.6%), compared to the wild-type strain. Conclusion: Bgl2p and Ecm33p contributed to the interaction between C. albicans and SHA beads. Deletion of these genes triggered overexpression of the ALS1 gene in the bgl2Δ/ecm33Δ mutant strain, and deletion of all three genes caused a significant decrease in adhesion. |
| Databáze: | OpenAIRE |
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